Addgene

We narrowed to 18,020 results for: Met

Showing: 401 - 420 of 18020 results

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B7-4

Plasmid

#111492

Purpose

Met-tRNA formyltransferase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Met-tRNA formyltransferase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS-M1A,M142A(KaganF62)

Plasmid

#52002

Purpose

Expresses the human MAVS CDS containing two point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)

Plasmid

#52018

Purpose

Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A,M142A(KaganE20)

Plasmid

#52011

Purpose

Expresses the human MAVS CDS containing the point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RNMT

Plasmid

#112705

Purpose

Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-GST-RNMT

Plasmid

#112709

Purpose

Expresses N-terminally GST-tagged human RNMT in bacterial cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

GST and thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro-{beta}4 siRNA

Plasmid

#16266

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SIRNA Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pSUPER.retro-{beta}4 SCR

Plasmid

#16267

Depositor

Arthur Mercurio

Article

Chung et al J Biol Chem. 2004 Jul 30. 279(31):32287-

Insert

SCR Beta 4 Integrin (ITGB4 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RNMT 1-120

Plasmid

#112706

Purpose

Expresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 121-476

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT 121-476

Plasmid

#112707

Purpose

Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 2-120

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pAc-His-hAR-2

Plasmid

#89091

Purpose

baculovirus transfer vector for histidine-tagged full-length human androgen receptor, starts Met-Ala-His6-Met-hAR

Depositor

Elizabeth Wilson

Article

Liao et al Biochemistry. 1999 Jul 27;38(30):9718-27

Insert

hAR (AR Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only
pMSCV-NLS-CBRed-d2-hygro

Plasmid

#139196

Purpose

non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBRed

Use

Luciferase and Retroviral

Tags

d2 PEST domain and nuclear localization signal (N…

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-NLS-CBGreen-FIRE-puro

Plasmid

#139195

Purpose

Luminescent reporter of ERK activity

Depositor

Matthew Lazzara

Article

Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

Insert

CBG99

Use

Luciferase and Retroviral

Tags

Fra1 PEST domain fragment (FIRE) and nuclear loca…

Expression

Mammalian

Mutation

See depositor comments below

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Pin1

Plasmid

#40773

Depositor

Dustin Maly

Insert

Pin1 (PIN1 Human)

Use

Tags

6His and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

T7

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ΔN-RNMT

Plasmid

#112708

Purpose

Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Use

Tags

FLAG and HIV Rev nuclear export signal (NES)

Expression

Mammalian

Mutation

deleted amino acids 2-120, mutated active-site D2…

Promoter

CMV

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJ128

Plasmid

#19962

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pJ131

Plasmid

#29485

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only
HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pJ158

Plasmid

#29492

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pJ177

Plasmid

#29494

Depositor

Jonathan Tait

Article

Jin et al J Biol Chem 2004; 279:40351-57.

Insert

annexin V-128 (ANXA5 Human)

Use

Tags

(Met)-Ala-Gly-Gly-Cys-Gly-His

Expression

Bacterial

Mutation

Deletion of the initiator Met at position 1 of wi…

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only