We narrowed to 14,176 results for: TIM

1. human EB1-GFP mutagenized K148 K150 K151 all converted to A (JB144)

   Plasmid

   #39305

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   K148A, K150A and K151A

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

   Plasmid

   #39306

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   E211A, K212A, E213A, R214A, D215A and Y217A

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)

   Plasmid

   #39307

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T249A, D250A and E251A

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

4. CHyMErA LbCas12a Nuclease Optimization hgRNA Library

   Pooled Library

   #209734

   Purpose

   CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different LbCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

5. CHyMErA AsCas12a Nuclease Optimization hgRNA Library

   Pooled Library

   #209735

   Purpose

   CHyMErA Cas12a Nuclease Optimization hgRNA Libraries allows assessment and comparison of the efficacy of different AsCas12a nucleases in the context of the Cas9-Cas12a CHyMErA system.

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Expression

   Mammalian

   Use

   CRISPR and Lentiviral

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

6. Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone

   Pooled Library

   #1000000075

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of mouse genes

   Depositor

   Feng Zhang

   Available Since

   March 17, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1

   Pooled Library

   #1000000100

   Purpose

   CRISPR gRNA knockout pooled library (version 1) for targeting the human genome

   Depositor

   Eric Lander, David Sabatini

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

8. Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA

   Pooled Library

   #1000000096

   Purpose

   CRISPR gRNA knockout pooled library for targeting the mouse genome

   Depositor

   Eric Lander, David Sabatini

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

9. Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

   Pooled Library

   #1000000095

   Purpose

   CRISPR gRNA knockout pooled library for targeting the human genome

   Depositor

   Eric Lander, David Sabatini

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (AAV5)

    Viral Prep

    #209655-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (#209655). In addition to the viral particles, you will also receive purified pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag plasmid DNA. CAG-driven, Cre-dependent expression of the high affinity ATP biosensor iATPSnFR2. These AAV preparations are suitable purity for injection into animals.

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Promoter

    CAG

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag (AAV5)

    Viral Prep

    #209658-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag (#209658). In addition to the viral particles, you will also receive purified pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag plasmid DNA. CAG-driven, Cre-dependent expression of cpSFGFP.HaloTag (non-responsive control). These AAV preparations are suitable purity for injection into animals.

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Promoter

    CAG

    Tags

    SFGFP

    Available Since

    Oct. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

12. pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209652

    Purpose

    Expression of iATPSnFR2, medium affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95K.HaloTag
    

    Use

    AAV

    Mutation

    A95K

    Promoter

    CAG

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209651

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Mutation

    S29W.A95K

    Promoter

    CAG

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209653

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Mutation

    A95A.A119L

    Promoter

    CAG

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.CAG.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209654

    Purpose

    Non-responsive control

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV.CAG.(cyto).iGlucoSnFR2.mIRFP670nano3

    Plasmid

    #244072

    Purpose

    Cytosolic expression of green glucose sensor with non-responsive mIRFP

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2.mIRFP670nano3
    

    Use

    AAV

    Tags

    mIRFP670nano3

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.CAG.(ER).iGlucoSnFR2.HaloTag

    Plasmid

    #244073

    Purpose

    ER expression of green glucose sensor with non-responsive HaloTag

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2.HaloTag
    

    Use

    AAV

    Tags

    Calreticulin and HaloTag

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV.CAG.(mem).iGlucoSnFR2

    Plasmid

    #244077

    Purpose

    Plasma membrane targeted expression of green glucose sensor

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2
    

    Use

    AAV

    Tags

    IgG kappa leader

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.CAG.(ER).iGlucoSnFR2.H348H.HaloTag

    Plasmid

    #244074

    Purpose

    ER expression of green glucose sensor (high affinity) with non-responsive HaloTag

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2.H348H.HaloTag
    

    Use

    AAV

    Tags

    Calreticulin and HaloTag

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.CAG.(cyto).iGlucoSnFR2.H348H.HaloTag

    Plasmid

    #244070

    Purpose

    Cytosolic expression of green glucose sensor (high affinity) with non-responsive HaloTag

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2.H348H.HaloTag
    

    Use

    AAV

    Tags

    HaloTag

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge