We narrowed to 3,649 results for: WOL

1. pegRNA_HEK4_20-7

   Plasmid

   #201974

   Purpose

   Express a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology arm

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   pegRNA_HEK4_20-7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO1-puro-U6-sgRNA-TGM2

   Plasmid

   #69239

   Purpose

   U6 driven SpCas9 sgRNA expression for TGM2 site

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TGM2 sgRNA (TGM2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LbCas12a-DR-GC-swap-cr in pBCSK+

   Plasmid

   #126643

   Purpose

   Cloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a Full Length Direct Repeat GC Swap crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

   Plasmid

   #107317

   Purpose

   Expresses SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9
   

   Use

   CRISPR

   Tags

   3xHA and 2xNLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-21a_2xNLS_FnCpf1_MBP_protein_expression

   Plasmid

   #120801

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   2xNLS_FnCpf1
   

   Use

   CRISPR

   Tags

   MBP and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69227

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

   Plasmid

   #69225

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS2 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. FnoCas12a-DR-cr in PBCSK-

   Plasmid

   #126642

   Purpose

   Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SauCas9KKH CCR5-nicking sgRNA

   Plasmid

   #169863

   Purpose

   SauCas9KKH nicking sgRNA for CCR5

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9KKH CCR5-nicking sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69226

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. FnoCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126644

    Purpose

    Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

    Plasmid

    #69224

    Purpose

    Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS and Zif268

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-FLAG-SZT2 2241-end

    Plasmid

    #87039

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AA 2241-end

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-FLAG-SZT2 1-1021

    Plasmid

    #87036

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AA 1-1021

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS

    Plasmid

    #69223

    Purpose

    Expresses R1335K mutant SpCas9 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-HA-SZT2 2241-end

    Plasmid

    #87040

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 2241-end

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PBB-79

    Plasmid

    #8813

    Depositor

    Cynthia Wolberger

    Article

    Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

    Insert

    GABPb1 (1-157) (Gabpb1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

18. EW265 SFFV-mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP (FLP-IN)

    Plasmid

    #236124

    Purpose

    Plasmid encoding fluorescent reporter for PUF-9R binding which expresses mCherry constitutively and EGFP upon stop codon deamination due to ADAR-PUF9R binding

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    mCherry-SGc(stem-1(3xPUF9R-tar))cSG-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP

    Plasmid

    #236134

    Purpose

    Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal promoter with four ZNF35/ZNF250 bindi…

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EW408 (ZNF35tar-compact)x8-H2B-GFP

    Plasmid

    #236129

    Purpose

    Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF35 binding sites upstream

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal promoter with eight ZNF35 binding sit…

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge