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pJMC-2-Reverse

Plasmid

#197801

Purpose

Level 1 Module 2 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDTnLAPP2A frame 2

Plasmid

#28228

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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Hv_HPT:pCER6#2:Gus_introns

Plasmid

#215200

Purpose

Evaluating guard cell specific promotors in Barley

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

3-ketoacyl-coa synthase6

Use

Synthetic Biology

Expression

Plant

Promoter

HvCER6#2

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PLA2G4A-2

Plasmid

#184736

Purpose

cPLA2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human cPLA2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJMC-2-BsaI

Plasmid

#197818

Purpose

Level 1 module 2 with BsaI expansion point

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-2-Esp3I

Plasmid

#197830

Purpose

Level 1 module 2 with Esp3I expansion point

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Ssh1 gRNA#2

Plasmid

#163395

Purpose

Cas9-mediated knockout of Ssh1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh2 gRNA#2

Plasmid

#163398

Purpose

Cas9-mediated knockout of Ssh2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh2 (Ssh2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ssh3 gRNA#2

Plasmid

#163401

Purpose

Cas9-mediated knockout of Ssh3 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Ssh3 (Ssh3 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-2

Plasmid

#164859

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-2

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-2

Plasmid

#164866

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-2

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGeneSwitch

Plasmid

#125216

Purpose

Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGAL80

Plasmid

#125219

Purpose

Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL80

Use

Other

Tags

T2A

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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HM110 prp16-2

Plasmid

#111277

Purpose

prp16-2 in pSE358(Trp)

Depositor

Christine Guthrie

Article

Madhani et al Genetics. 1994 Jul;137(3):677-87.

Insert

PRP16

Expression

Yeast

Mutation

prp-2 (D575N)

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pYU-2-Env

Plasmid

#133997

Purpose

Env gene from HIV-1 YU-2 in pcDNA3.1 expression vector

Depositor

Himanshu Garg

Article

Joshi et al J Immunol. 2016 Feb 15;196(4):1768-79. d

Insert

Envelope Glycoprotein

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-2

Plasmid

#204200

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3

Expression

Mammalian

Mutation

silent mutations

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-2

Plasmid

#129727

Purpose

Expressing SpCas9 and sgAAVS1-2

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-2

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-shBRCA1 #2

Plasmid

#44595

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

BRCA1 (Brca1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
RUBY-ABE#2

Plasmid

#232176

Purpose

T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.

Depositor

Yiping Qi

Article

Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

Insert

2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold

Use

CRISPR

Tags

3xflag and NLS

Expression

Plant

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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