We narrowed to 8,982 results for: ef1;

1. pBS598 EF1alpha-EGFPcre

   Plasmid

   #11923

   Purpose

   Expresses EGFP-Cre fusion under the control of the EF1alpha promoter; EGFP is human codon-optimized.

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   EGFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1-Pls3-sfGFP

   Plasmid

   #205750

   Purpose

   Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.

   Depositor

   Jerome Bonnet

   Article

   Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

   Type

   Empty backbone

   Use

   Shuttle vector gram+ gram-

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pls3 (Phyperspank mutant, IPTG inducible)

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

   Plasmid

   #194458

   Purpose

   CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

   Depositor

   Ana Sofia Coroadinha

   Article

   Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

   Insert

   Anti-CD19 CAR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-iCreV

   Plasmid

   #140135

   Purpose

   Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iCreV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-CCreV

   Plasmid

   #140132

   Purpose

   can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Insert

   CCreV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-NCreV

   Plasmid

   #140131

   Purpose

   can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Insert

   NCreV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-dCasRx-RBM25

   Plasmid

   #221001

   Purpose

   Transient expression of dCasRx-RBM25. EF1a promoter.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1α-Cre-2A-Puro

   Plasmid

   #207743

   Purpose

   Expression of the Cre recombinase linked to a puro selectable marker

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   Cre-2A-Puro
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pT3-EF1a-c-Met

   Plasmid

   #31784

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-sRGECO

    Plasmid

    #137125

    Purpose

    Viral expression of sRGECO

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    sRGECO
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E217D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_EF1a_flagLaM4-Notch-Gal4VP64

    Plasmid

    #216669

    Purpose

    Expresses anti-mCherry synNotch Gal4VP64

    Depositor

    Leonardo Morsut

    Insert

    anti-mCherry synNotch Gal4VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PL-SIN-EF1α-EGFP

    Plasmid

    #21320

    Purpose

    Control Lentiviral vector with strong EF1a promoter, expresses EGFP

    Depositor

    James Ellis

    Article

    Hotta et al Nat Methods. 2009 May . 6(5):370-6.

    Insert

    Enhanced Green Fluorescence Protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3-EF1a-Bmi1

    Plasmid

    #31783

    Depositor

    Xin Chen

    Article

    Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

    Insert

    Bmi1 (BMI1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Flpo

    Plasmid

    #55637

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV2

    Insert

    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-EF1aL-nlsGFP-W

    Plasmid

    #126688

    Purpose

    Constitutive, ubiquitous, nuclear-localized GFP expression.

    Depositor

    Darrell Kotton

    Article

    Tang et al Nat. Phys. 18, 1371–1378 (2022).

    Insert

    nlsGFP
    

    Use

    Lentiviral

    Tags

    6xHIS and NLS

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3-EF1a-YapS127A

    Plasmid

    #46049

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

18. JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

    Plasmid

    #233538

    Purpose

    A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF1a-BirA-V5-neo

    Plasmid

    #100548

    Purpose

    Expresses C-terminal V5-tagged BirA biotin ligase

    Depositor

    Jian Xu

    Article

    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

    Insert

    C-terminal V5-tagged BirA
    

    Use

    Tags

    V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-EF1a-Backbone(NG)

    Plasmid

    #27963

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TAL-Backbone(NG)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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