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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,323 results for: ttl

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Showing: 401 - 420 of 5323 results

  6. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Article
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available Since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80424-plasmid-map-sequence-id-151441

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  9. pCMV-dCas13-inactive M3M14nes

    Plasmid
    #157855
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157855-plasmid-map-sequence-id-307628

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  15. pP150

    Plasmid
    #113201
    Purpose
    shuttle vector, N-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Dictyostelium discoideum shuttle vector, n-termin…
    Tags
    mTurquoise2
    Available Since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    113201-plasmid-map-sequence-id-217569

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  16. pENTR_mCherry

    Plasmid
    #139554
    Purpose
    mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    mCherry
    Expression
    Mammalian
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139554-plasmid-map-sequence-id-274727

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  17. pENTR-rtTA3G

    Plasmid
    #139556
    Purpose
    rtTA ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    rtTA3G
    Expression
    Mammalian
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139556-plasmid-map-sequence-id-274724

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  18. pENTR-Emerald

    Plasmid
    #139549
    Purpose
    Emerald ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Emerald
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139549-plasmid-map-sequence-id-274625

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  19. pENTR-TetR

    Plasmid
    #139558
    Purpose
    TetR ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    TetR
    Expression
    Mammalian
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139558-plasmid-map-sequence-id-274733

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  20. pENTR-Venus

    Plasmid
    #139551
    Purpose
    Venus ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    Venus
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139551-plasmid-map-sequence-id-274619

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Showing: 401 - 420 of 5323 results