We narrowed to 59,070 results for: {{{s}}}

1. pPaxillin-Electra2

   Plasmid

   #184926

   Purpose

   Expresses paxillin fused to blue fluorescent protein Electra2

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   paxillin-Electra2
   

   Use

   Tags

   Electra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 882 pSG5L HA PTEN G129E

   Plasmid

   #10746

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G129E

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFLAG_attP

   Plasmid

   #110095

   Purpose

   Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 884 pGEX2T PTEN G129E

   Plasmid

   #10747

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G129E

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

5. 819 pGEX2T PTEN C124S

   Plasmid

   #10745

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C124S

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

6. HA-RIPLET ΔRING

   Plasmid

   #218506

   Purpose

   RIPLET lacking N-terminal RING domain in pcDNA3.1

   Depositor

   Sun Hur

   Article

   Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

   Insert

   RIPLET delta RING domain (RNF135 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SpRY

   Plasmid

   #198544

   Purpose

   Expresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMito-Electra1

   Plasmid

   #179481

   Purpose

   Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   COX8A-Electra1
   

   Use

   Tags

   Electra1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEThT-CAHS1

   Plasmid

   #90027

   Purpose

   To express His-tagged CAHS1 in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   CAHS1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK010

    Plasmid

    #69874

    Purpose

    expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

    Depositor

    Georg Nagel, Robert Kittel

    Article

    Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

    Insert

    Channelrhodopsin-2
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    D156C (XXL)

    Promoter

    Drosophila Hsp70

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMA_Int

    Plasmid

    #110096

    Purpose

    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Insert

    mycophage integrase
    

    Use

    Required for transformation of attb integrating v…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 817 pSG5L HA PTEN G129R

    Plasmid

    #10748

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G129R

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pCas9-VRER_2A_GFP

    Plasmid

    #75475

    Purpose

    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

    Depositor

    Marc Tessier-Lavigne

    Article

    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

    Insert

    Cas9-VRER
    

    Use

    CRISPR

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    CAG

    Available since

    May 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEThT-SAHS2

    Plasmid

    #90026

    Purpose

    To express His-tagged SAHS2 in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    SAHS2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p6643 MSCV-P C-FlagHA 31E7-Kozak

    Plasmid

    #35021

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV31 E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 6, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pTol2-nbt-Electra1-P2A-mScarlet

    Plasmid

    #196113

    Purpose

    zebrafish expression of nbt-Electra1-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra1-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pActin-Electra1

    Plasmid

    #184941

    Purpose

    beta-actin labelling with blue fluorescent protein Electra1

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-beta-Actin
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCytERM-Electra1

    Plasmid

    #179476

    Purpose

    ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-Electra1
    

    Use

    Tags

    Electra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAN147

    Plasmid

    #129202

    Purpose

    yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p6647 MSCV-P C-Flag HA 2aE7

    Plasmid

    #35137

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    HPV2a E7
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only