We narrowed to 4,363 results for: pes;

1. AAV-Syn-TRPV1-P2A-DsRed

   Plasmid

   #200830

   Purpose

   Transduce all neuron types with AAV to express TRPV1 along with a DsRed fluorescence reporter

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-DsRed (Trpv1 Rat)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fireworks RFP[PTC+] (AVA2626)

   Plasmid

   #85444

   Purpose

   Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

   Depositor

   Joan Steitz

   Article

   Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

   Insert

   tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
   

   Use

   Minimal backbone fragment for low-copy bacterial …

   Expression

   Mammalian

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUAST-attB-3xHA-V5-RpL10Ab

   Plasmid

   #125223

   Purpose

   3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

   Depositor

   Herman Dierick

   Article

   Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

   Insert

   RpL10Ab (RpL10Ab Fly)
   

   Tags

   3x HA, V5

   Expression

   Insect

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-TRS-Leader-SARS-CoV-2-EGFP

   Plasmid

   #171585

   Purpose

   Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   TRS-Leader-SARS-CoV-2-d2eGFP
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-CH115gp160-QES.c14-754*

   Plasmid

   #123282

   Purpose

   Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (CH115) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Premature stop co…

   Promoter

   CMV

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-7xTcf-NLS-ONL-CP

   Plasmid

   #65716

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
   

   Use

   Luciferase

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2-7xTcf-NLS-YNL-CP

   Plasmid

   #65714

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
   

   Use

   Luciferase

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2-7xTcf-NLS-CNL-CP

   Plasmid

   #65715

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
   

   Use

   Luciferase

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgFFL_3x_siRNA

   Plasmid

   #59893

   Purpose

   synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites

   Depositor

   Georg Seelig

   Article

   Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

   Insert

   mCherry with intron containing the mouse mir-124–3
   

   Tags

   PEST and VAMP 3 UTR

   Expression

   Mammalian

   Mutation

   VAMP 3 UTR--has three siRNA-like targets (fully c…

   Promoter

   doxycycline (Dox)-inducible promoter (CMV/TO)

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-DIO-ECS-dSpyCatcher003

    Plasmid

    #234963

    Purpose

    Cre-dependent ECS-facing destabilized SpyCatcher003 (facing the extracellular space)

    Depositor

    Hajime Hirase

    Article

    Vittani et al bioRxiv 2025.03.18.643859

    Insert

    ECS-dSC
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCOA-Leu-FvPPT1

    Plasmid

    #179923

    Purpose

    Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Insert

    FvPPT1
    

    Expression

    Yeast

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Promoter

    Yarrowia lipolytica EXP1

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.15_pT-REX17

    Plasmid

    #195497

    Purpose

    Firefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Promoter region of lncRNA T-REX17
    

    Tags

    luc2-hPEST

    Expression

    Mammalian

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

    Plasmid

    #182918

    Purpose

    Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

    Depositor

    Daniel Gerlich

    Article

    Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

    Insert

    pH2B (H2BC16P Human)
    

    Tags

    mNeonGreen-FRB(DmrC)

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-CH111-QES.c13.L544Y-754*

    Plasmid

    #123264

    Purpose

    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4-AD8-QES.i08.c04-754*

    Plasmid

    #123258

    Purpose

    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Premature stop co…

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-SHIV327C-HuA10-QES.i09.c07

    Plasmid

    #123269

    Purpose

    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    SHIV327C-Hu A10 Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations V208M, …

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgFFL_d23

    Plasmid

    #59892

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--3rd and fourth seed site removed

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgFFL_d3

    Plasmid

    #59891

    Purpose

    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.

    Depositor

    Georg Seelig

    Article

    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

    Insert

    mCherry with intron containing the mouse mir-124–3
    

    Tags

    PEST and VAMP 3 UTR

    Expression

    Mammalian

    Mutation

    VAMP 3 UTR--3rd seed site removed

    Promoter

    doxycycline (Dox)-inducible promoter (CMV/TO)

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. hZMPSTE24-3FLAG_pQCXIP

    Plasmid

    #89759

    Purpose

    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.

    Depositor

    Martin Dorf

    Article

    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127

    Insert

    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    

    Use

    Retroviral; Bicistronic expression: puromycin re…

    Tags

    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…

    Expression

    Mammalian

    Mutation

    Y253H in ZMPSTE24 (see depositor comments below)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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