We narrowed to 5,308 results for: pcDNA3.1+

1. pcDNA3 proHB-EGF NC

   Plasmid

   #11605

   Depositor

   Rosalyn Adam

   Article

   Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

   Insert

   HB-EGF (HBEGF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

   Promoter

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

2. CoV2-N-S202R

   Plasmid

   #177950

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (S202R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S202R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. beta Synuclein-VC

   Plasmid

   #89484

   Purpose

   expresses human beta Synuclein fused to the C-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

   Insert

   SNCB (SNCB Human)
   

   Use

   Tags

   c-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. SP-GFP-CD274-NU

   Plasmid

   #121139

   Purpose

   Mammalian expression of GFP tagged CD274

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Inserts

   CD274 (CD274 Human)
   CD274 (CD274 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-EMTB-TurboID-V5

   Plasmid

   #190736

   Purpose

   Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Tags

   Myc, TurboID, and V5

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CoV2-N-P199L

   Plasmid

   #177949

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P199L) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P199L

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KRas FAK biosensor

   Plasmid

   #78301

   Purpose

   FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

   Insert

   KRas FAK biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-N-mSOG-FRB-IRES-mCherry

   Plasmid

   #136602

   Purpose

   Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   N-mSOG-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hGATA-1 WT

   Plasmid

   #118352

   Purpose

   This plasmid expresses human GATA-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PME-1 pcDNA-StrepIII

    Plasmid

    #119292

    Purpose

    Mammalian overexpression of StrepII-tagged full length PPME1

    Depositor

    Jukka Westermarck

    Article

    Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

    Insert

    PPME1 (PPME1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #159281

    Purpose

    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    AsCpf1-HA-2A-GFP
    

    Use

    Multicistronic vector with both caggs promoter-dr…

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1-dmito-oROS-HT

    Plasmid

    #216418

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    dmito-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. VN-beta Synuclein

    Plasmid

    #89483

    Purpose

    expresses human beta Synuclein fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

    Insert

    SNCB (SNCB Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. alpha catenin conformation sensor

    Plasmid

    #71709

    Purpose

    This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    alpha-catenin FRET Sensor
    

    Use

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. hTPST2

    Plasmid

    #11253

    Depositor

    Hyeryun Choe

    Article

    Choe et al Cell. 2003 Jul 25. 114(2):161-70.

    Insert

    TPST2 (TPST2 Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HiBiT-FZD7

    Plasmid

    #195845

    Purpose

    Expresses human FZD7 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD7 (FZD7 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. β₂AR-mCFP

    Plasmid

    #38260

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    β₂AR (ADRB2 Human)
    

    Use

    Tags

    Flag, mCFP, and signal peptide sequence (MKTIIALS…

    Expression

    Mammalian

    Mutation

    diglycine linker (replacing terminator codon)

    Promoter

    CMV

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. CoV2-N-T205I

    Plasmid

    #177954

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (T205I) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T205I

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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