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We narrowed to 7,360 results for: ust

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Showing: 4221 - 4240 of 7360 results

  7. pT3EF1aH-myr-Akt

    Plasmid
    #179909
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.
    Depositor
    Xin Chen
    Article
    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:
    Insert
    Akt1 (Akt1 Mouse)
    Tags
    HA and Myr
    Expression
    Mammalian
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    179909-plasmid-map-sequence-id-357764

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  9. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  11. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  13. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  17. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available Since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

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  18. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

    Plasmid
    #237479
    Purpose
    Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (TMEM106B Human)
    Use
    Lentiviral
    Tags
    P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    M210A + F213A
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    237479-plasmid-map-sequence-id-476565

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  19. pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

    Plasmid
    #223134
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223134-plasmid-map-sequence-id-447700

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  20. pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

    Plasmid
    #223135
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223135-plasmid-map-sequence-id-447701

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Showing: 4221 - 4240 of 7360 results