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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 8481 - 8520 of 160365 results

  2. pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA

    Plasmid
    #113688
    Purpose
    SaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Insert
    SaCas9 (NEWENTRY )
    Use
    AAV and CRISPR
    Tags
    Flag, HAx2, KASH, and NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113688-plasmid-map-sequence-id-231828

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  3. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  4. pTF-ACE2s

    Plasmid
    #162786
    Purpose
    The extracellular domain of Angiotensin converting enzyme 2 (ACE2) expression
    Depositor
    Ivan Vorobiev
    Article
    Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    10xHis, FLAG, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alphaā€¦
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    162786-plasmid-map-sequence-id-333795

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Showing: 8481 - 8520 of 160365 results