We narrowed to 17,418 results for: SHE

1. pKK1 -pFlp-18::mito4x-GCaMP6f

   Plasmid

   #194969

   Purpose

   Fluorescent reporter of mitochondrial matrix Ca2+.

   Depositor

   Frederic Hoerndli

   Insert

   4 repeats of Cox8 presequence plus GCaMP6f
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Flp-18

   Available since

   June 21, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pMZ376

   Plasmid

   #74213

   Purpose

   Combined sgRNA/Cas9 vector with KanMX marker

   Depositor

   Mikel Zaratiegui

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-(neg27)GFP-GGS9-Cre-6xHis

   Plasmid

   #89253

   Purpose

   Expresses GFP with -27 theoretical charge fused to Cre in E.Coli

   Depositor

   David Liu

   Insert

   (neg27)GFP-GGS9-Cre-6xHis
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. sCFP in pBSDONR P4r-P2

   Plasmid

   #121530

   Purpose

   For making C-terminal sCFP fusions using multisite Gateway cloning

   Depositor

   Roger Innes

   Insert

   sCFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUltra-Smurf-luc-RTTA3

   Plasmid

   #59438

   Purpose

   silence doxycyclin inducible pUltra-dox (also expresses luciferase)

   Depositor

   Kitai Kim, Yildirim Dogan

   Inserts

   luciferase
   Reverse TET-Transactivator 3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   loxP5171

   Promoter

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-RSV-jGCaMP7s-W

   Plasmid

   #126690

   Purpose

   Mammalian calcium sensor protein.

   Depositor

   Darrell Kotton

   Insert

   jGCaMP7s
   

   Use

   Lentiviral

   Tags

   6xHIS, T7, Xpress

   Expression

   Mutation

   A52V, A317L

   Promoter

   RSV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV Tor1a-V5

   Plasmid

   #175135

   Purpose

   Lentiviral expression of Tor1a-Myc

   Depositor

   Larry Gerace

   Insert

   Tor1a (Tor1a Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-RSV-jGCaMP7f-W

   Plasmid

   #126691

   Purpose

   Mammalian calcium sensor protein.

   Depositor

   Darrell Kotton

   Insert

   jGCaMP7f
   

   Use

   Lentiviral

   Tags

   6xHIS, T7, Xpress

   Expression

   Mutation

   H78K, A317L

   Promoter

   RSV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBluescript mouse ptc

   Plasmid

   #58699

   Purpose

   full length mouse ptc cDNA

   Depositor

   Matthew Scott

   Insert

   ptc (Ptch1 Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pMC6-TEFkFLP(LYS2)

    Plasmid

    #49458

    Purpose

    overespression of Flp step-arrest mutant in yeast, LYS2 selection

    Depositor

    Marc Gartenberg

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    TEF1

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK266

    Plasmid

    #73326

    Purpose

    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    violacein biosynthesis protein VioD
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIC81

    Plasmid

    #63065

    Purpose

    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    PMFI, IMAGE clone 6148250 (PMF1 Human)
    

    Use

    Tags

    EYFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid

    #133575

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PTEN-VC

    Plasmid

    #89332

    Purpose

    expresses hPTEN fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    PTEN1 (PTEN Human)
    

    Use

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. B0030m_BC

    Plasmid

    #74394

    Purpose

    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]

    Depositor

    Douglas Densmore

    Insert

    RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBtet-Bla/GFP

    Plasmid

    #198221

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP

    Depositor

    Eric Davis

    Insert

    EGFP
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK690

    Plasmid

    #72380

    Purpose

    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)

    Depositor

    T. J. Kappock

    Insert

    glutaredoxin-4
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2B CFP

    Plasmid

    #198059

    Purpose

    mCerulean fused to human H2B protein

    Depositor

    Amro Hamdoun

    Insert

    H2B
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mCerulean

    Expression

    Mutation

    Promoter

    sp6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK409

    Plasmid

    #72423

    Purpose

    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK405

    Plasmid

    #72418

    Purpose

    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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