We narrowed to 5,068 results for: cre encoding plasmid

1. pET28b-SpRY-His

   Plasmid

   #179320

   Purpose

   Plasmid for bacterial expression and purification of SpRY-Cas9

   Depositor

   Julián Cerón, Miguel Angel Moreno-Mateos

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-nSpCas9-evoCDA1

   Plasmid

   #209042

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   evoCDA1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-nSpCas9-APOBEC3A

   Plasmid

   #209040

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC3A-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

   Plasmid

   #163179

   Purpose

   Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

   Plasmid

   #163176

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

   Plasmid

   #163177

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

   Plasmid

   #163178

   Purpose

   Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS1401

   Plasmid

   #29198

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEMS1538

   Plasmid

   #29261

   Purpose

   High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   Ple67 (FEV Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pLenti-nSpCas9-NoCBE

    Plasmid

    #209038

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-nSpCas9-APOBEC1

    Plasmid

    #209039

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-nSpCas9-BEACON2

    Plasmid

    #209041

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    BEACON2-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W

    Plasmid

    #163147

    Purpose

    Lentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    CHAT (CHAT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W

    Plasmid

    #163175

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

    Plasmid

    #163174

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W

    Plasmid

    #163170

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W

    Plasmid

    #163172

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCsA

    Plasmid

    #136067

    Purpose

    Acceptor plasmid for even level position 1, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. GFP-CaM-uTEVp

    Plasmid

    #163032

    Purpose

    FLiCRE protease for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP-CaM-uTEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRE:mCherry-p2a- bReaChES

    Plasmid

    #163037

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    mCherry-p2a-bReaChES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge