Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 11,784 results for: emb

Clear keywords
Showing: 4241 - 4260 of 11784 results

  6. AKR7A3

    Plasmid
    #38862
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKR7A3 (Akr7a3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pCAG-nucGFP11-3xControlgRNA

    Plasmid
    #224568
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Publication
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224568-plasmid-map-sequence-id-451496

    Enlarge

  10. pET21b-MBPx-MBPx-SpyTag003

    Plasmid
    #216291
    Purpose
    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
    Depositor
    Mark Howarth
    Publication
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MBPx-MBPx-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 and SpyTag003
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216291-plasmid-map-sequence-id-443903

    Enlarge

  14. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

    Enlarge

  16. pRB051_LEU2

    Plasmid
    #196993
    Purpose
    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.
    Depositor
    Matthew Maurano
    Publication
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    196993-plasmid-map-sequence-id-394871

    Enlarge

  17. pMito-dCherry-FRB

    Plasmid
    #186573
    Purpose
    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells
    Depositor
    Stephen Royle
    Publication
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Insert
    Dark MitoTrap
    Expression
    Mammalian
    Mutation
    Mutation of "K70N" to render mCherry no…
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    186573-plasmid-map-sequence-id-368442

    Enlarge

Showing: 4241 - 4260 of 11784 results