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pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri-U6-Camk2d sgRNA7

Plasmid

#220127

Purpose

Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadA

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauriCas9

Use

AAV

Tags

HA tag

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7

Plasmid

#220129

Purpose

Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSauri Cas9C

Use

AAV and CRISPR

Expression

Mammalian

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

Plasmid

#226915

Purpose

Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSp Cas9N, inteinN

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

Plasmid

#226916

Purpose

Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSp Cas9C

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7

Plasmid

#226678

Purpose

Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauri Cas9N, inteinN

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TMD20

Plasmid

#163675

Purpose

Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD21

Plasmid

#163676

Purpose

Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD22

Plasmid

#163677

Purpose

Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD24

Plasmid

#163678

Purpose

Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.2

Plasmid

#149431

Purpose

miniTdg gene, SUMOylation mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.1

Plasmid

#149430

Purpose

miniTdg gene, catalytic mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

N151A catalytic mutant, SNPs of Tdg gene in OLA/1…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PIWIL2 ts2

Plasmid

#115870

Purpose

PIWIL2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PIWIL2 shRNA (PIWIL2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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MPP5 gRNA (BRDN0001148199)

Plasmid

#76943

Purpose

3rd generation lentiviral gRNA plasmid targeting human MPP5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MPP5 (PALS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MPP5 gRNA (BRDN0001147829)

Plasmid

#76944

Purpose

3rd generation lentiviral gRNA plasmid targeting human MPP5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MPP5 (PALS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MPP5 gRNA (BRDN0001147491)

Plasmid

#76945

Purpose

3rd generation lentiviral gRNA plasmid targeting human MPP5

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MPP5 (PALS1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPN089

Plasmid

#91614

Purpose

Express sgRNA targeting human FURIN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

FURIN (FURIN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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