We narrowed to 10,196 results for: Uty

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pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

Plasmid

#216694

Purpose

Synchronize trafficking of GPI-AP from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
SBP-mApple-FIREtag-GPI

Tags

FIREtag, IL2 signal sequence, Streptavidin Bindin…

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA

Plasmid

#205318

Purpose

All-in-one AAV vector expressing ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISDge10 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-DEST47-SIRT1

Plasmid

#242131

Purpose

Expression in mammalian cells

Depositor

J. Carl Barrett, Izumi Horikawa

Article

Michishita et al Mol Biol Cell. 2005 Oct;16(10):4623-35.

Insert

SIRT1 (SIRT1 Human)

Tags

Cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

Plasmid

#40810

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(31-51), S218E, S222D

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax-NRTH

Plasmid

#136922

Purpose

Expresses ABEmax-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-GFP-AURKA-mCherry

Plasmid

#99876

Purpose

Expression of GFP-AURKA-mCherry for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP and mCherry

Expression

Bacterial

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHM024Env

Plasmid

#137691

Purpose

Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

Depositor

Anjali Joshi

Article

Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

Insert

HIV-1 Envelope Glycoprotein

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG eGFP T2A SP HALO HAPLN1

Plasmid

#228200

Purpose

AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

Depositor

Sungjin Park

Article

Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

Insert

HAPLN1 (Hapln1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

constitutive CAG promoter

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (P#468)

Plasmid

#14607

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (CCNT1 Human)

Tags

GST

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pDawn-Ag43

Plasmid

#107742

Purpose

Expresses Ag43 under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

Ag43 (flu )

Expression

Bacterial

Mutation

removed all pstI cut sites with silent mutations,…

Promoter

pLambda

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMP71-super-IL-2

Plasmid

#174599

Purpose

Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SaCas9 TadCBEd

Plasmid

#193845

Purpose

Express TadCBEd (with SaCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd with SaCas9

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET41-MBP-t-A3A-t-His

Plasmid

#109231

Purpose

Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

Depositor

Rahul Kohli

Article

Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

Insert

human APOBEC3A (APOBEC3A Human, Optimized for E. coli expression)

Tags

His and MBP

Expression

Bacterial

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pHEE901-A3A

Plasmid

#229044

Purpose

nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

Depositor

Huawei Zhang

Article

Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_Thy1StTA

Plasmid

#97411

Purpose

TET driver for amplified expression in cortical neurons

Depositor

Tetsuo Yamamori

Article

Sadakane et al Cell Rep. 2015 Dec 1;13(9):1989-99. doi:

Insert

tTA

Use

AAV

Expression

Mammalian

Promoter

mouse thy1PSs

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-TSF-TEVcs-ULK1

Plasmid

#171416

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ heiCas9

Plasmid

#186929

Purpose

For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

Depositor

Joachim Wittbrodt

Article

Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

Insert

myc-flexible linker-oNLS-Cas9-oNLS

Use

CRISPR

Tags

myc-flexible linker-optimizedNLS and optimizedNLS

Expression

Mammalian

Promoter

sp6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2 K509A

Plasmid

#85120

Purpose

yeast expression of mutant EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K509A

Promoter

GAL1

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-STAT1

Plasmid

#82722

Purpose

Expresses human STAT1

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

STAT1 (STAT1 Human)

Tags

3xFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only