We narrowed to 9,276 results for: ef1

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pFETCh_TEAD1

Plasmid

#86291

Purpose

Donor vector for 3' FLAG tag of human TEAD1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

TEAD1 homology arms (TEAD1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCSN069

Plasmid

#166728

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Article

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-Mxi1

Use

CRISPR

Tags

Mxi1

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAN1935

Plasmid

#127203

Purpose

Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1

Use

Synthetic Biology

Available Since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pYC25

Plasmid

#63914

Purpose

Recipient overexpressing vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYC16

Plasmid

#63913

Purpose

Recipient overexpressing vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

CFP

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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Flag‐Tim2

Plasmid

#49213

Purpose

expresses Flag tagged Tim2

Depositor

Lawrence Kane

Article

Knickelbein et al J Immunol. 2006 Oct 15;177(8):4966-70.

Insert

Tim2 (Timd2 Mouse)

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

no Tim2 signal sequence; R80L and S196D compared …

Promoter

EF1a

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only
AKAP13

Plasmid

#73230

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

AKAP13 (AKAP13 Human)

Tags

His-6-TEV

Expression

Bacterial

Mutation

pfam00621:RhoGEF 2002-2193, pfam00169:PH 2236-2336

Promoter

T7

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only
nSpG UGI Puro

Plasmid

#235046

Purpose

Control nickase Cas9

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pFC482

Plasmid

#246439

Purpose

The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal

Depositor

Ron Weiss

Article

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

CasE

Tags

NLS

Expression

Mammalian

Mutation

SapI domesticated

Promoter

EF-1a

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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IQ-compete reporter (integrating)

Plasmid

#247176

Purpose

Plasmid for genomic integration of the IQ-compete reporter (GFP-TCS-Quencher-CL1) into the YPRCΔ15 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

GFP-TCS-Quencher-CL1

Expression

Yeast

Promoter

TEF1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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IQ-compete reporter (episomal)

Plasmid

#247217

Purpose

Single-copy plasmid expressing the IQ-compete reporter (GFP-TCS-Quencher-CL1).

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

GFP-TCS-Quencher-CL1

Expression

Yeast

Promoter

TEF1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pJL233

Plasmid

#243693

Purpose

Stably expresses a chimeric protein, ALDH18A1(1-97aa)-SUPV3L1 (108aa-358aa)-ALDH18A1 (351-795aa)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

ALDH18A1(1-97aa)-SUPV3L1 (108aa-358aa)-ALDH18A1 (351-795aa)

Use

Lentiviral

Promoter

EF1a

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJL192

Plasmid

#243691

Purpose

Stably expresses a chimeric protein, SUPV3L1(1-114aa)-ALDH18A1(106aa-385aa)-SUPV3L1(351-786aa)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

SUPV3L1(1-114aa)-ALDH18A1(106aa-385aa)-SUPV3L1(351-786aa)

Use

Lentiviral

Promoter

EF1a

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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SpG-IP

Plasmid

#242073

Purpose

Expresses Cas9-SpG in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpG

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJL191

Plasmid

#243690

Purpose

Stably expresses a chimeric protein, SUPV3L1(1-199aa)-ALDH18A1(106aa-385aa)-SUPV3L1(451-786aa)

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

SUPV3L1(1-199aa)-ALDH18A1(106aa-385aa)-SUPV3L1(451-786aa)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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L4872 VP64-intN in PolyTX-mNeonGreen

Plasmid

#244191

Purpose

Constitutive expression of VP64-intN split synTF component

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

Split synTF component VP64-intN

Use

Synthetic Biology

Tags

3xFLAG - nuclear localization sequence

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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L4873 intC-ZF9 in PolyTX-mTagBFP2

Plasmid

#244192

Purpose

Constitutive expression of intC-ZF9 split synTF component

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

Split synTF component intC-ZF9

Use

Synthetic Biology

Tags

nulcear localization sequence - HA

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_033

Plasmid

#210755

Purpose

PU_Zea mays EF1a promoter

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Zea Mays Elongation factor 1A promoter

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pILFPZ1A9

Plasmid

#223835

Purpose

Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

Insert

pTEF1>mTagBFP>mNeonGreen>

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pDIV302

Plasmid

#204973

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
nat1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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