We narrowed to 11,709 results for: Chia

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS TIR-2 sfGFP

Plasmid

#154464

Purpose

pET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSIMcpf1

Plasmid

#153034

Purpose

For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSIMcpf1

Plasmid

#153034

Purpose

For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lwa_Cas13a_WT

Plasmid

#91865

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LwaCas13a

Use

CRISPR

Tags

His6-MBP-TEV site

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pPBCAG-rtTAM2-IH

Plasmid

#140827

Purpose

Expresses rtTAM2-IRES-HygR in mammalian cells

Depositor

Hideyuki Okano

Article

Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

Inserts

rtTAM2
HygR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPBCAG-rtTAM2-IH

Plasmid

#140827

Purpose

Expresses rtTAM2-IRES-HygR in mammalian cells

Depositor

Hideyuki Okano

Article

Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

Inserts

rtTAM2
HygR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTF-lacZ

Plasmid

#153036

Purpose

For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

CRISPR array

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTF-lacZ

Plasmid

#153036

Purpose

For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

CRISPR array

Expression

Bacterial

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCas12f1

Plasmid

#171182

Purpose

Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

Cas12f1

Use

CRISPR

Expression

Bacterial

Promoter

cas9 promoter from Streptococcus pyogenes

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCas12f1

Plasmid

#171182

Purpose

Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

Cas12f1

Use

CRISPR

Expression

Bacterial

Promoter

cas9 promoter from Streptococcus pyogenes

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTrc-trGPPS(CO)-LS

Plasmid

#50603

Purpose

pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

Depositor

Jay Keasling

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Inserts

Limonene Synthase
ApR
LacIq

Use

Synthetic Biology

Expression

Bacterial

Mutation

truncated codon optimized geranylpyrophosphate sy…

Promoter

Trc

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pTrc-trGPPS(CO)-LS

Plasmid

#50603

Purpose

pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

Depositor

Jay Keasling

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Inserts

Limonene Synthase
ApR
LacIq

Use

Synthetic Biology

Expression

Bacterial

Mutation

truncated codon optimized geranylpyrophosphate sy…

Promoter

Trc

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCA24N_6xHis-TEV-rpsA

Plasmid

#83585

Purpose

Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

rpsA (rpsA Escherichia coli)

Tags

His tag

Expression

Bacterial

Promoter

p-T5 Lac

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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