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CamKII-mtPyronicSF

Plasmid

#228906

Purpose

Expression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

pyronic SF

Use

AAV

Tags

Cox8 targetting

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV GFP

Plasmid

#213685

Purpose

Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag

Depositor

Thomas Kukar

Article

Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

Insert

Twin-Strep GFP

Use

AAV

Tags

Twin-Strep

Promoter

cytomegalovirus enhancer/chicken β-actin promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Stargazin-EBFP2-FRB

Plasmid

#222417

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

Cacng2 (Cacng2 Mouse)

Tags

EBFP2-HA-FRB

Expression

Mammalian

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 genomic A. thaliana PLDGAMMA3

Plasmid

#209188

Purpose

pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT4G11840 (PLDGAMMA3 Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-GlcisPT

Plasmid

#203172

Purpose

Yeast expression vector for Giardia cis-prenyltransferase

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

UPPS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Control V5-Twist

Plasmid

#201376

Purpose

Expresses Twist for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPIP1;4-NLS-3xmCitrine

Plasmid

#192524

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

PLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

PIP1;4

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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ptAD-seq-ubi63E-Gal4-DBD

Plasmid

#111930

Purpose

Vector to express tAD candidates from a library cloned from fragmented transcription factor coding sequences fused to the Gal4 DNA binding domain.

Depositor

Alexander Stark

Article

Arnold et al EMBO J. 2018 Aug 15;37(16). pii: embj.20

Insert

tAD-seq Gal4-DBD

Expression

Insect

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-ZNF354C_mutant_M41K-L42K-E43K

Plasmid

#194185

Purpose

Expresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Oo et al Sci Rep. 2020 Nov 5;10(1):19079. doi: 10

Insert

ZNF354C (ZNF354C Human)

Tags

Myc-DDK

Expression

Mammalian

Mutation

KRAB domain mutant M41K/L42K/E43K

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pST_D4NWF2_DrHD_b03_dom3 (pBS0681)

Plasmid

#185207

Purpose

For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

D4NWF2_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_UPI0005D02939_DrHD_b03_dom3 (pBS0684)

Plasmid

#185210

Purpose

For the mammalian expression of the Deinococcus radiodurans protein UPI0005D02939_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

UPI0005D02939_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_P06727_DrHD_b03_dom3 (pBS0683)

Plasmid

#185209

Purpose

For the mammalian expression of the Deinococcus radiodurans protein P06727_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

P06727_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q1XI24_DrHD_b03_dom3 (pBS0682)

Plasmid

#185208

Purpose

For the mammalian expression of the Deinococcus radiodurans protein Q1XI24_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q1XI24_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK7_DrHD_b03_dom3 (pBS0679)

Plasmid

#185206

Purpose

For the mammalian expression of the Deinococcus radiodurans protein H2FLK7_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK7_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_A8E631_DrHD_b03_dom1 (pBS0678)

Plasmid

#185205

Purpose

For the mammalian expression of the Deinococcus radiodurans protein A8E631_DrHD_b03_dom1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A8E631_DrHD_b03_dom1

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK7_DrHD_b03_dom1 (pBS0668)

Plasmid

#185203

Purpose

For the mammalian expression of the Deinococcus radiodurans protein H2FLK7_DrHD_b03_dom1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK7_DrHD_b03_dom1

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_A0A1I1U3P5_DrHD_b03_dom3 (pBS0665)

Plasmid

#185202

Purpose

For the mammalian expression of the Deinococcus radiodurans protein A0A1I1U3P5_DrHD_b03_dom3. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1I1U3P5_DrHD_b03_dom3

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DR_1172 (pBS0313)

Plasmid

#185170

Purpose

For the mammalian expression of the Deinococcus radiodurans protein DR_1172. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DR_1172

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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