We narrowed to 13,099 results for: shRNA

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pORE303N_CEN12Fuzzy3MYB

Plasmid

#194440

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, Fuzzy3MYB

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS1

Plasmid

#174301

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS2

Plasmid

#174302

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN1

Plasmid

#194439

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA targeting CEN1

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS3

Plasmid

#174303

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

Depositor

Lee Lynd

Article

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

Plasmid

#188975

Purpose

A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g1 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target2 (Mpphot)

Plasmid

#186727

Purpose

Gateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA_Target1 (Mpphot)

Plasmid

#186726

Purpose

Gateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

sgRNA_Mphot

Expression

Bacterial

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-mGreenLantern332

Plasmid

#188483

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

PB_rtTA_BsmBI

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV152

Plasmid

#188485

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV162

Plasmid

#188486

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-CMV51

Plasmid

#188484

Purpose

gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

CMV promoter sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

Plasmid

#73542

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

Plasmid

#73543

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4E

Plasmid

#176868

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG5

Plasmid

#176869

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_5

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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