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pCMV-bpNLS-eeBxb1 (CJT555)

Plasmid

#248209

Purpose

eeBxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-eeBxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

eeBxb1(V74A, E229K, V375I)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-T7-sgRNA

Plasmid

#245118

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-FLAG-La-Cas9

Plasmid

#245102

Purpose

Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

3xFLAG-La-Cas9

Use

CRISPR

Tags

3xFLAG-La

Mutation

codon optimized for T. brucei

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-T7-sgRNA

Plasmid

#245108

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-zFucci-pA-exorh-GFP (JDW 1489)

Plasmid

#242593

Purpose

A Tol2 expression vector containing the zebrafish Ubi promoter driving zFUCCI to label cell cycle state. Exorh-EGFP in the backbone to label pineal cells.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin, mCherry-zCdt1

Use

Zebrafish expressio

Promoter

zebrafish Ubi

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3HA::tbTurboID::3HA

Plasmid

#247590

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tbTurboID

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6 CHIP delU-box-Myc

Plasmid

#246738

Purpose

Mammalian expression of human CHIP with U-box domain deleted, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

deletion of U-box domain

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TK310

Bacterial Strain

#196340

Purpose

Bacterial strain lacking adenlyate cyclase (cyaA, needed for cAMP synthesis) and phosphodiesterase (cpdA, which degrades cAMP); unable to control its intracellular cAMP levels

Depositor

Terence Hwa, Thomas Kuhlman

Article

Kuhlman et al Proc Natl Acad Sci U S A. 2007 Apr 3;104

Bacterial Resistance

None

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only