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PRKD2 gRNA (BRDN0001145881)

Plasmid

#77616

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD2 (PRKD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-1058

Plasmid

#135347

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058-UGI

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-DDRGK1-K-less-HA

Plasmid

#139849

Purpose

Lentiviral expression of human DDRGK1-K-less-HA

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

DDRGK1 (DDRGK1 Human)

Use

Lentiviral

Tags

HA

Mutation

K116R, K120R, K121R,K124R, K128R, K146R, K176R, K…

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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miniSOG-Keratin-17

Plasmid

#57770

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PCRII apoEa

Plasmid

#121900

Purpose

Plasmid for making zebrafish apoEa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoEa (apoea Zebrafish)

Use

Unspecified

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

Plasmid

#139482

Purpose

Expresses PhyB621-mCherry-HRasCT in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

PhyB621 (PHYB Mustard Weed)

Use

Lentiviral

Tags

mCherry-HRasCT

Expression

Mammalian

Mutation

G229L and deletion of aa Y at position 235 in mch…

Promoter

Tet responsible element

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)( full )-pcw107-V5

Plasmid

#64645

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
PRKD2 gRNA (BRDN0001147619)

Plasmid

#77615

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKD2 (PRKD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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tdEos-Keratin-17

Plasmid

#57629

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Keratin (KRT18 Human)

Tags

tdEos

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
TPD52L3 gRNA (BRDN0001145193)

Plasmid

#76952

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPD52L3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TPD52L3 (TPD52L3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)( full )-pcw107

Plasmid

#64644

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
mIFP-Keratin-17

Plasmid

#56225

Purpose

Note- This plasmid contains Keratin 18. Localization: Intermediate Filaments, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Keratin (KRT18 Human)

Tags

mIFP

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBabe mouse Bmf

Plasmid

#17241

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
Tau BA 12->10 N296N

Plasmid

#203383

Purpose

For transfection and in vitro assay

Depositor

Stefan Stamm

Article

Margvelani et al Biochim Biophys Acta Mol Basis Dis. 2024

Insert

MAPT (MAPT Human)

Expression

Mammalian

Mutation

N296N (silent mutation)

Promoter

CMVf

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-T453D

Plasmid

#235209

Purpose

Expresses GRP78 with T453D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Threonine 453 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-R197H

Plasmid

#235208

Purpose

Expresses GRP78 with R197H mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Arginine 197 to Histidine

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-G227D

Plasmid

#235207

Purpose

Expresses GRP78 with G227D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Glycine 227 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CCM2_d410-444_eGFP

Plasmid

#208877

Purpose

Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids deletion : 410-444

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus R200W

Plasmid

#209179

Purpose

Mammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent protein

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (R200W) fused with Venus (NKX6-2 Human)

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (R200W)

Promoter

CMV

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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