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DDX5

Plasmid

#155523

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX5 (DDX5 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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CD109-bio-His

Plasmid

#51862

Purpose

Expresses full-length CD109 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CD109 (CD109 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-AKAR4

Plasmid

#181957

Purpose

Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-AKAR4 (AKAP5 Human)

Tags

AKAP79, Cerulean, HIS tag, and cpVenus(E172)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a mNeonGreen-3K-B1-10-IRES-mKate2-2A-PuroR

Plasmid

#215377

Purpose

Lentiviral expression of a cytosolic-localized split mNeonGreen variant that enables efficient complementation when the missing beta strand is present. mKate2 is present as a transduction control.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

mNeonGreen-3K-1-10

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcU6_3 MS2 sgRNA

Plasmid

#92394

Purpose

Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.3

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherryC2-Gephyrin P1

Plasmid

#68820

Purpose

mCherry tagged Geph for mammalian expression

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

Insert

Gephyrin (Gphn Rat)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-β-catenin_S23A,T40A,T41A,T112A

Plasmid

#190824

Purpose

For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

β-catenin (CTNNB1 Chicken)

Tags

GFP

Expression

Mammalian

Mutation

S23A, T40A, T41A, T112A

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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CLK1

Plasmid

#155458

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK1 (CLK1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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ITGAV-bio-His

Plasmid

#51919

Purpose

Expresses full-length Integrin alpha-V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITGAV (ITGAV Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLL5.0 mCherry-hCortKD-Flcortmut3YD

Plasmid

#187266

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mCherry

Mutation

3YD (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YF mCherry

Plasmid

#187265

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

mCherry

Mutation

3YF (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

Plasmid

#193728

Purpose

Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

Depositor

Colin Akerman

Article

Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

Insert

ClopHensorN

Use

AAV

Tags

Strep-II (N terminal on insert)

Expression

Mammalian

Promoter

EF1a

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-VC

Plasmid

#110648

Purpose

expresses a PAR-binding (PBZ) domain fused to venus C-terminus

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

HA and VC

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBX_Sec61b_smGFP_Myc

Plasmid

#236092

Purpose

Constitutively encodes Sec61b-smGFP-Myc (sfGFP-based spaghetti monster fluorescent protein embedding Myc epitope tags), for targeting the endoplasmic reticulum.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Sec61b-smGFP-Myc (SEC61B Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_CBP-GFP

Plasmid

#221926

Purpose

Dox-inducible expression of CBP-GFP in mammalian cells by retroviral transduction

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Use

Retroviral

Tags

GFP

Mutation

insertion of CBP-GFP

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-pTau nanobody (A2) with sortase tag

Plasmid

#233218

Purpose

Mammalian epression of anti-pTau nanobody (A2) with a sortase tag for direct dye conjugation.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv [Preprint]. 2025 Feb 20:2023.10.

Insert

Anti-pTau nanobody (A2)

Tags

Sortase tag, His tag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBX_RPS2_smGFP_V5

Plasmid

#236088

Purpose

Constitutively encodes RPS2-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting the 40S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPS2-smGFP-V5 (RPS2 Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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