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We narrowed to 10,128 results for: Met

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Showing: 4321 - 4340 of 10128 results

  2. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

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  3. pTRE3G-human cGASdeltaN R255E-HA

    Plasmid
    #186894
    Purpose
    Doxycycline-dependent expression of human cGAS gene (with R255E mutation) in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Publication
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with R255E mutation and C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    HA
    Mutation
    N-terminal truncation, R255E
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186894-plasmid-map-sequence-id-366485

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  4. pTetOne-FLAG-human cGASdeltaN-HA

    Plasmid
    #186899
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Publication
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Synthetic, Human)
    Use
    Retroviral
    Tags
    FLAG and HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186899-plasmid-map-sequence-id-366534

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  5. AAV-CMV-scFVDnmt3AL_bGHpA

    Plasmid
    #177347
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177347-plasmid-map-sequence-id-353618

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  6. AAV-CMV-scFVtet2_bGHpA

    Plasmid
    #177352
    Purpose
    AAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 2 (TET2 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    Catalytic domains of human TET2 (1129–1936 and 14…
    Promoter
    CMV promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177352-plasmid-map-sequence-id-353673

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  8. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

    Plasmid
    #174637
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Publication
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174637-plasmid-map-sequence-id-346992

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  9. pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)

    Plasmid
    #174636
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Publication
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) (Kif1a Synthetic, Mouse)
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174636-plasmid-map-sequence-id-346990

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  10. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid
    #122003
    Purpose
    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast
    Depositor
    Brian Kuhlman
    Publication
    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d
    Insert
    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    mVenus-FLAG
    Expression
    Yeast
    Mutation
    Set2 point mutations K538G, K539S, R549G, K550S, …
    Promoter
    ADH1
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    122003-plasmid-map-sequence-id-238611

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Showing: 4321 - 4340 of 10128 results