We narrowed to 19,663 results for: Met

Showing: 4321 - 4340 of 19663 results

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pSCALPS-mAgo2-EGFP

Plasmid

#209935

Purpose

Lentiviral expression of mouse Argonaute-2 in mammalian cells

Depositor

Phillip Zamore

Article

Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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mRuby2-CENPB-N-22

Plasmid

#55893

Purpose

Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CENPB (CENPB Human)

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTYB11-PTBP1-RRM1

Plasmid

#89153

Purpose

Recombinant expression of PTBP1-RRM1 in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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mOrange2-Golgi-7

Plasmid

#57959

Purpose

Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Golgi (B4GALT1 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-mCherry-attP(C31)-*BleoR

Plasmid

#183752

Purpose

phiC31 landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

FRT-mCherry-attP-BleoR-F3

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST cyto Grx1-roGFP2

Plasmid

#64994

Purpose

for expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGPT2_5

Plasmid

#163455

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 5 targeting GPT2 (GPT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag1-3HABeta2-mEos2

Plasmid

#114187

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Article

Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Tags

3HA, FLAG, and mEOS2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-mCherry

Plasmid

#35508

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2

Plasmid

#170853

Purpose

AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-Myr-PTEN

Plasmid

#206983

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

N-terminal myristoylation site

Expression

Mammalian

Promoter

tet-on promoter

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

Plasmid

#98932

Purpose

Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Clover-TOMM20-N-10

Plasmid

#56307

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

TOMM20 (TOMM20 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MERS-coV-NSP3-c001

Plasmid

#173085

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))

Tags

mCerulean

Expression

Bacterial

Mutation

D1109-D1275, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEB31 (hANGPTL8-V5-His lenti)

Plasmid

#109116

Purpose

V5 and His-tagged human ANGPTL8 in a lentiviral vector

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (ANGPTL8 Human)

Use

Lentiviral

Tags

6x His and V5

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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mIFP-H2B-6

Plasmid

#56223

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H2B (H2BC11 Human)

Tags

mIFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

Plasmid

#98931

Purpose

Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1 and AAV5

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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mRuby2-TOMM20-N-10

Plasmid

#55913

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

TOMM20 (TOMM20 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pUAST mito roGFP2-Grx1

Plasmid

#64995

Purpose

for expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1_G3

Plasmid

#86611

Purpose

Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via hdr using ouabain

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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