We narrowed to 5,339 results for: pcdna3.1

1. nSaCas9 I744 miniABEMax V82G

   Plasmid

   #135379

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (I744 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 I744 miniABEMax V82G
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. V5-bArr1

   Plasmid

   #201491

   Purpose

   Expression of the beta-arrestin1 containing a N-terminal V5 tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   V5-bArr1 (ARRB1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Rs4084-RBD-8h

   Plasmid

   #161825

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Rs4231-RBD-8h

   Plasmid

   #161824

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T320-D518)

   Promoter

   CMV

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE3646

   Plasmid

   #137714

   Purpose

   Expresses LbCas12a RVRR variant in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   LbCas12a-RVRR
   

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HCMVgO-sfGFP (Merlin)

   Plasmid

   #136452

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Tags

   Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-MED29

   Plasmid

   #15409

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

   Insert

   MED29 (MED29 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. Opto-GPR3

   Plasmid

   #106000

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR3 (GPR3 Human, Bovine)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CoV2-N-D3L

   Plasmid

   #177945

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D3L) (N )
   

   Expression

   Mammalian

   Mutation

   D3L

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid

    #136445

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-717). Codon-optimize…

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CoV2-N-A119S

    Plasmid

    #177948

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (A119S) (N )
    

    Expression

    Mammalian

    Mutation

    A119S

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLAG-MED8

    Plasmid

    #15406

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED8 (MED8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-Cx43

    Plasmid

    #60691

    Purpose

    mammalian expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-p160C

    Plasmid

    #46

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    expresses aa580-1330

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

    Plasmid

    #132560

    Purpose

    Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

    Inserts

    ABEmax
    sgRNA, with Tetraloop replaced by com aptamer
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-f:PGC1(delta CTD)

    Plasmid

    #1030

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PGC1a (Ppargc1a Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta CTD

    Available Since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-D2s-L-V2

    Plasmid

    #19968

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-C terminal Venus (DRD2 Aequorea Victoria, Human)
    

    Tags

    C terminal Venus and Flag

    Expression

    Mammalian

    Available Since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)

    Plasmid

    #183254

    Purpose

    Expression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Article

    Muhsin-Sharafaldine et al Oncotarget. 2016 Aug 30;7(35):56279-5629

    Insert

    Tissue factor (F3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CoV2-N-M234I

    Plasmid

    #177955

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (M234I) (N )
    

    Expression

    Mammalian

    Mutation

    M234I

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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