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pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

Plasmid

#237479

Purpose

Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

TMEM106B (TMEM106B Human)

Use

Lentiviral

Tags

P2A-Blasticidin

Expression

Mammalian

Mutation

M210A + F213A

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE3 mutant

Plasmid

#133310

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE3 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

ccacgtgacc changed to cAaAAAAacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

Plasmid

#202553

Purpose

Nontargeting control vector with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Mutation

Puromycin-resistance gene in the pLV hU6-sgRNA hU…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MultiSite Gateway plasmid toolKit

Plasmid Kit

#1000000050

Purpose

Cloning kit for constructing fully-customizable three-part fusion proteins, based on MultiSite Gateway from Life Technologies. 12 promoter-less Gateway entry clones, N- and C-term flanking modules

Depositor

Tomas Santalucia

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
RAS mutant clone collection

Plasmid Kit

#1000000089

Purpose

Wild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clones

Depositor

Dominic Esposito

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only
Fungal Toolkit for Modular Cloning (FTK)

Plasmid Kit

#1000000191

Purpose

Consists of 96 genetic parts as Modular Cloning-compatible entry vectors, including synthetic and native promoters, terminators, selection markers, and CRISPR components, for filamentous fungi.

Depositor

Arnold Driessen

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP5(nth-ydgR)

Bacterial Strain

#110245

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
TB205 △trpC

Bacterial Strain

#230038

Purpose

Fluorescently labelled (mCherry) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
TB204 △trpC

Bacterial Strain

#230037

Purpose

Fluorescently labelled (GFP) E. coli, auxotrophic for tryptophan

Depositor

Martin Ackermann, Olga Schubert

Article

Dal Co et al Nat Ecol Evol. 2020 Mar;4(3):366-375. do

Bacterial Resistance

None

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP4(essQ-cspB)

Bacterial Strain

#110244

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP6(ygcE-ygcF)

Bacterial Strain

#110246

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_rLP1(atpI-gidB)

Bacterial Strain

#110241

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP3(ybbD-ylbG)

Bacterial Strain

#110243

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655_fLP2(yieN-trkD)

Bacterial Strain

#110242

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
TB204

Bacterial Strain

#230033

Purpose

Fluorescently labelled (monomeric sfGFP) E. coli

Depositor

Tobias Bergmiller, Calin Guet

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
TB201

Bacterial Strain

#230031

Purpose

Fluorescently labelled (YFP) E. coli

Depositor

Tobias Bergmiller, Calin Guet

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
ATMW-BL21

Bacterial Strain

#218769

Purpose

E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

Bacterial Resistance

Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

Species

Escherichia coli

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only
pMOTag2T

Plasmid

#26295

Depositor

George Cross

Type

Empty backbone

Use

Trypanosome in-situ tagging vector

Tags

Ty-1

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pMOTag2H

Plasmid

#26296

Depositor

George Cross

Type

Empty backbone

Use

Trypanosome in-situ tagging vector

Tags

Triple HA

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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Showing: 4321 - 4340 of 9811 results

Results per page:

Sort results by:

We narrowed to 9,811 results for: try

pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

pGL4-eIF3B MBE3 mutant

pGL4-eIF3B MBE2 mutant

pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

MultiSite Gateway plasmid toolKit

RAS mutant clone collection

Fungal Toolkit for Modular Cloning (FTK)

E. coli K-12 MG1655_rLP5(nth-ydgR)

TB205 △trpC

TB204 △trpC

E. coli K-12 MG1655_fLP4(essQ-cspB)

E. coli K-12 MG1655_rLP6(ygcE-ygcF)

E. coli K-12 MG1655_rLP1(atpI-gidB)

E. coli K-12 MG1655_fLP3(ybbD-ylbG)

E. coli K-12 MG1655_fLP2(yieN-trkD)

TB204

TB201

ATMW-BL21

pMOTag2T

pMOTag2H

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