We narrowed to 6,787 results for: PROC
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Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
289aa Met133, 138, 186Ileu ELL2
Plasmid#127272PurposeExpresses 289aa human ELL2 with Met133, 138, 186IleuDepositorInsertELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
ExpressionMammalianMutationMet133, 138, 186 Ileu, cut with XbaI blunt fillinPromoterpEF1aAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B G120A no-stop
Plasmid#123206PurposeGateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneMutationGlycine 120 to Alanine, stop codon removedAvailable SinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-GABARAPL1 G116
Plasmid#123119PurposeExpresses EGFP-GABARAPL1 G116 in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
TagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsEGFP and mCherryExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B G120A
Plasmid#123235PurposeExpresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
TagsEGFP and mCherryExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable SinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs PQR>ala
Plasmid#83319Purposehuman DNA-PKcs PQR>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
ExpressionMammalianMutation5 phosphorylation sites in the PQR cluster substi…Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs ABCDE>A
Plasmid#83318Purposehuman DNA-PKcs/ABCDE>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
ExpressionMammalianMutation6 phosphorylation sites in the ABCDE cluster subs…Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-LC3B G120
Plasmid#123241PurposeExpression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acids 121-125. Stop codon after G12…PromoterPGKAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APPSw;T719N
Plasmid#177094PurposeFor lentiviral transduction of APPsw; T719NDepositorInsertAPPsw;T719N (APP Human)
UseLentiviralMutationT719N, K651N and M652L (please see depositor comm…Available SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDyn1
Plasmid#64067PurposeHuman cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).DepositorInsertDYNC1H1 (DYNC1H1 Human)
UseCre/LoxTagsHis-ZZ-TCS-SNAPfExpressionInsectMutationCodon optimized for Sf9Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLXC2B-PLAG1-3xFLAG
Plasmid#228057Purposelentiviral expression of human PLAG1 with 3xFLAG tagDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
ExpressionMammalianAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-LC3B G120A
Plasmid#123242PurposeExpression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterPGKAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116
Plasmid#123243PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterPGKAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
R979-X58-008: Hs.KRAS4b(1-188)
Plasmid#159691PurposeBaculovirus protein expression of KRAS4b (1-188)DepositorInsertHs.KRAS4b(1-188) (KRAS Human)
ExpressionInsectAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-APP751
Plasmid#193772PurposeEntry vector for human APP isoform having 751 amino acid residuesDepositorInsertAPP (APP Human)
Available SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116A
Plasmid#123245PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationGlycine 116 to AlaninePromoterPGKAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3B-G120A-GFP
Plasmid#123108PurposeExpresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
Tags3xFLAG and EGFPExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRET_PST-C
Plasmid#228570PurposeExpressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of PancreastatinDepositorInsertChromogranin-A (CHGA Human)
TagsmCitrine and mScarlet-IExpressionMammalianMutationFRET sensor; see explanatory notePromotercmvAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRET_PST-N
Plasmid#228571PurposeExpressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of PancreastatinDepositorInsertChromogranin-A (CHGA Human)
TagsmCitrine and mScarlet-IExpressionMammalianMutationFRET sensor; see explanatory notePromotercmvAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRET_CST-C
Plasmid#228572PurposeExpressed a FRET sensor for determining proteolytic cleavage of the C-terminal side of CatestatinDepositorInsertChromogranin-A (CHGA Human)
TagsmCitrine and mScarlet-IExpressionMammalianMutationFRET sensor; see explanatory notePromotercmvAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FRET_CST-N
Plasmid#228573PurposeExpressed a FRET sensor for determining proteolytic cleavage of the N-terminal side of CatestatinDepositorInsertChromogranin-A (CHGA Human)
TagsmCitrine and mScarlet-IExpressionMammalianMutationFRET sensor; see explanatory notePromotercmvAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
HR700_HA2TP53_Ex5
Plasmid#228860PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 5DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex6
Plasmid#228861PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 6DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex7
Plasmid#228862PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 7DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HR700_HA2TP53_Ex8
Plasmid#228863PurposeBackbone for generation of donor vectors for CRISPR/Cas9 mediated Saturation Genome Editing (SGE) of TP53 Exon 8DepositorTypeEmpty backboneUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceFeb. 4, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET41-FeoB-OL2del
Plasmid#216742PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [LEDSGYMA…Available SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only