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pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AS1371A

Plasmid

#224409

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AWLDLE

Plasmid

#224410

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 WT

Plasmid

#185632

Purpose

expression of GST-tagged wild-type PML B-box1 domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 C4C4

Plasmid

#185640

Purpose

expression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

H155C H161C

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 W157E

Plasmid

#185641

Purpose

expression of GST-tagged mutant PML B-box1 W157E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

W157E

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML B-box1 F158E

Plasmid

#185642

Purpose

expression of GST-tagged mutant PML B-box1 F158E domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

F158E

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

Plasmid

#216170

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

Plasmid

#216172

Purpose

Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- Control- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

Plasmid

#216173

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-H3(pLxIS-PLPLR)

Plasmid

#221287

Purpose

Expression of H3 (pLxIS-PLPLR) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

H3 (pLxIS-PLPLR) (H3 Synthetic)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

K to Q mutations made to inhibit NLS

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-L326A/V336A/H338A/Y358A

Plasmid

#215895

Purpose

Expression of TRBP-L326A/V336A/H338A/Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A/V336A/H338A/Y358A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB3 CTF-FLAG

Plasmid

#217683

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB3 (BAI3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-856

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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SEC61B-2Strep

Plasmid

#210375

Purpose

Expresses SEC61B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SEC61B (SEC61B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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HT121_Fsyn_QuasAr6a(Q3_codon)_citrine

Plasmid

#208606

Purpose

Expressing QuasAr6a_Citrine with an alternate codon

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

QuasAr6a

Use

Lentiviral

Tags

Citrine

Promoter

hSyn

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-sCAG-GluClα.CreON

Plasmid

#196995

Purpose

Cre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.

Depositor

David Bennett

Article

Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

Insert

GluClα -Cerulean

Use

AAV and Cre/Lox

Tags

Cerulean

Promoter

short CAG

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP1a-withDmTD_H

Plasmid

#146504

Purpose

Mammalian Expression of HsDcp1a-withDmTD

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a-withDmTD (DCP1A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-withDmTD_H

Plasmid

#146506

Purpose

Mammalian Expression of HsDCP1a-withDmTD

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-withDmTD (DCP1A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut1_G

Plasmid

#146408

Purpose

Bacterial Expression of HsDCP1a_539-582-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582-mut1 (DCP1A Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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