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pJH2829

Plasmid

#199693

Purpose

Pcex-1 tomm-20::miniSOG UrSL mCherry unc-54 3' UTR C.elegans RIM neuron expression of miniSOG RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

miniSOG

Tags

RFP

Expression

Worm

Promoter

Pcex-1

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-LGP2-Myc

Plasmid

#215874

Purpose

LGP2-Myc expression vector for overexpression

Depositor

Kumiko Ui-Tei

Article

Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

Insert

LGP2 (DHX58 Human)

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43 mRuby K84ONBK

Plasmid

#216226

Purpose

TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

Transactive response DNA binding protein of 43 kDa (TARDBP Human)

Expression

Mammalian

Mutation

Lysine 84 mutated to a TAG stop codon

Promoter

CMV Promoter

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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2x3APWWP_K299E_D333A-IFP2.0C

Plasmid

#215753

Purpose

Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP_K299E_D333A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ERBB3-NTEV-tcs-GV-2xHA

Plasmid

#214611

Purpose

ERBB3 receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

ERBB3-NTEV (ERBB3 Human)

Tags

TEV-Gal4-VP16-2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (R354H)

Plasmid

#197010

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

R354H

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (R221C)

Plasmid

#197009

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

R221C

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCA17-CRISPR-Tag (11XTS1) for dSpCas9

Plasmid

#199448

Purpose

CRISPR-Tag sequence that can be efficiently bound by dSpCas9

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

CRISPR-Tag

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R121Q)

Plasmid

#197007

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R121Q

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R354H)

Plasmid

#197006

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R354H

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (R221C)

Plasmid

#197005

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

R221C

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-SPOP-myc (BACK)

Plasmid

#197021

Purpose

Mammalian inducible expression vector for myc-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

Y353E

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_1-215opt_AB

Plasmid

#148367

Purpose

Bacterial Expression of CeeIF4E3_1-215

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_1-215

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_1-215opt-V58AI74A_AB

Plasmid

#148368

Purpose

Bacterial Expression of CeeIF4E3_1-215-V58AI74A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_1-215-V58AI74A

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-CeeIF4E3_30-215opt_AB

Plasmid

#148369

Purpose

Bacterial Expression of CeeIF4E3_30-215opt

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeeIF4E3_30-215opt

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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KBB438

Plasmid

#185092

Purpose

GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Expression

Yeast

Mutation

Rfa1 with amino acids 338-343 deleted

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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KBB372

Plasmid

#185084

Purpose

GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

Depositor

Ken Belanger

Article

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Mutation

Rfa1 amino acids 330-621

Promoter

GAL1

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R2

Plasmid

#179163

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

Insert

protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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