We narrowed to 8,106 results for: Dos;

1. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. tph-1(short)::rpl-22-HA

   Plasmid

   #122203

   Purpose

   C. elegans tagged ribosome expression plasmid

   Depositor

   Steven Flavell

   Article

   Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

   Insert

   tph-1(short)::rpl-22-HA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_YWHAG_14-3-3

   Plasmid

   #109905

   Purpose

   Protein expression and purification of YWHAG_14-3-3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   YWHAG_14-3-3 (YWHAG Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FZ-NbPDS

   Plasmid

   #184268

   Purpose

   pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.

   Depositor

   Feng Qu

   Article

   Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

   Insert

   CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   The protease processing site between MP and L-CP …

   Promoter

   CaMV 35S with duplicated enhancer

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Frankenbody-FLAG-Halo

   Plasmid

   #175296

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with HaloTag
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcw107

   Plasmid

   #62511

   Purpose

   Gateway Expression Vector

   Depositor

   David Sabatini, John Doench

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-N2-Frankenbody-HA

   Plasmid

   #175292

   Purpose

   Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-HA frankenbody fused with mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

   Plasmid

   #69623

   Purpose

   A FRET sensor for Galphai1 activation, encoded on a single plasmid.

   Depositor

   Dorus Gadella

   Article

   van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

   Inserts

   Gbeta-2A-cpV-Ggamma2
   Galphai-mTurquoise2
   

   Use

   Tags

   ciruclar permutated Venus (cp173V) and mTurquoise…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (IRES)

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPED

   Plasmid

   #162468

   Purpose

   Destination Expression vector for dicot plant prime editing

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Inserts

   Cas9H840A
   attR gateway destination cassette
   

   Use

   Tags

   MLV reverse transcriptase

   Expression

   Plant

   Mutation

   Change histidine 840 to Alanine based sequence o…

   Promoter

   2x35S

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-5X-1-Full-Length-MePCE

    Plasmid

    #113539

    Purpose

    Produces an N-terminal GST-tagged full length MePCE protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS_NP_EBOV

    Plasmid

    #103049

    Purpose

    Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    silent mutation (T to C) at position 928 of NP ge…

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-5X-1-LARP7

    Plasmid

    #113545

    Purpose

    Produces an N-terminal GST-tagged full length LARP7 protein.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    LARP7 (LARP7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMOS003-lenti-CMV-GCaMPer

    Plasmid

    #65227

    Purpose

    ER calcium sensor for lentivirus production

    Depositor

    Adam Cohen, Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    GCaMPer
    

    Use

    Lentiviral

    Tags

    circularly permuted GFP

    Expression

    Mammalian

    Mutation

    GCaMP3 (D324G, D360G, D397G, D435G)

    Promoter

    CMV

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTargeting_Mettl3C_FKBP-V

    Plasmid

    #165421

    Purpose

    Template for knockin FBKP-V into Mettl3 C-terminus

    Depositor

    Neil Brockdorff

    Article

    Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

    Insert

    Mettl3 C-terminus + linker + FKBP-V (Mettl3 Mouse)
    

    Use

    Mouse Targeting

    Tags

    FKBP-V

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSMP-EZH2_1

    Plasmid

    #36387

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH2 (EZH2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. p415 TEF Su9‐roGFP2‐Tsa2ΔCR

    Plasmid

    #83240

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For mitochondrial matrix expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    Su9-roGFP2-Tsa2dCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCr with Su9 targetting se…

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSC5

    Plasmid

    #188602

    Purpose

    Superior Conjugative Plasmid to Diverse Yeast

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-5'-ENGRAM

    Plasmid

    #179157

    Purpose

    piggyBac plasmid for 5' ENGRAM recorder

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minP

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. TPST2-EGFP

    Plasmid

    #66618

    Purpose

    Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)

    Depositor

    David Stephens

    Article

    Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

    Insert

    TPST2 (TPST2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert contains T370A relative to NM_001008566.1

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only