We narrowed to 30,799 results for: Ide

1. pX330-Flag-deSpCas9

   Plasmid

   #92114

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive eSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, K848A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFR-ET1

   Plasmid

   #44185

   Depositor

   Timothy Springer

   Article

   Mi et al Biochemistry. 2008 Sep 30;47(39):10314-2

   Insert

   EGFR-ET1 (EGFR Human)
   

   Tags

   3C cleavge site, protein C peptide tag, his6, SBP…

   Expression

   Mammalian

   Mutation

   I104V and D254N relative to NP_005219.2

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-mkgDUX4

   Plasmid

   #21156

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX4 (DUX4 Human)
   

   Expression

   Bacterial and Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRF+423Dux4

   Plasmid

   #21625

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   Double homeobox, chr 4 (DUX4 Human)
   

   Use

   Luciferase

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

   Plasmid

   #138144

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NES

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1P POLE1_1

   Plasmid

   #160762

   Purpose

   Suppress POLE1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLE1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDECKO_mCherry_non-targeting

   Plasmid

   #140109

   Purpose

   CRISPR-Cas9 library validation (negative control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

   Plasmid

   #201686

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1 (V106W)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-TRAIL-R4

    Plasmid

    #61385

    Purpose

    Expresses HA tagged TRAIL receptor 4

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 4 (TNFRSF10D Human)
    

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R4 leader peptide (aa1-29) replaced with TN…

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

14. INCTbiosyn-pAct-AtINTphiC31

    Plasmid

    #127535

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase phiC31.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase phiC31 coding sequence codon optimized for A. thaliana expression.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1P POLE1_2

    Plasmid

    #160764

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-TRAIL-R3

    Plasmid

    #61384

    Purpose

    Expresses TRAIL receptor 3

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 3 (TNFRSF10C Human)
    

    Tags

    HA tag and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R3 leader peptide (aa1-29) replaced with TN…

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. INCTbiosyn-pAct-AtINT13

    Plasmid

    #127534

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase 13.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 13 coding sequence codon optimized for A. thaliana expression.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21b GFP-Rab10 Q68LHis

    Plasmid

    #186015

    Purpose

    GFP-Rab10 Q68LHis

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    GFP-Rab10 Q68LHis (RAB10 Human)
    

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. gRNA-hIRF-1 #12/pSIR

    Plasmid

    #61080

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    gRNA_hIRF1 promoter #12 (IRF1 Human)
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. INCTbiosyn-pAct-AtINTBxb1

    Plasmid

    #127536

    Purpose

    Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
    

    Expression

    Plant

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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