We narrowed to 4,713 results for: LUM

1. LiRTX_ctrl

   Plasmid

   #214077

   Purpose

   Luminescent reporter system, NMD negative control. Intron present in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Remnant of linker sequence present at the end of …

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-Bip-GFP:V5:KDEL

   Plasmid

   #69917

   Purpose

   An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

   Depositor

   Kenneth Irvine

   Article

   Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

   Insert

   GFP
   

   Tags

   Drosophila BiP signal sequence, KDEL ER targeting…

   Expression

   Insect

   Promoter

   pMT

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPV-DsRed2-ER-5

   Plasmid

   #170570

   Purpose

   Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-DsRed2-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGWB402-er-mCherry

   Plasmid

   #186288

   Purpose

   Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

   Insert

   er-mCherry
   

   Expression

   Bacterial

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCerulean3-CytERM-N-17

   Plasmid

   #55412

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   CytERM
   

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDC32-Kar2-CFP-HDEL

   Plasmid

   #96989

   Purpose

   Express endoplasmic reticulum (ER) marker in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   Kar2-CFP-HDEL
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIgnaviCas9

   Plasmid

   #127595

   Purpose

   Expresses IgnaviCas9 in E. coli

   Depositor

   Stephen Quake

   Article

   Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

   Insert

   IgnaviCas9
   

   Tags

   6xHis Tag and Maltose binding protein

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRATIO3267

   Plasmid

   #141312

   Purpose

   Gateway cloning compatible dual bioluminescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   gLUC and redLUC

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRATIO1267

   Plasmid

   #141301

   Purpose

   Gateway cloning compatible dual bioluminescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   gLUC and redLUC

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQIq_MRGS_HIS8_(27G2)_FLAG

    Plasmid

    #199616

    Purpose

    Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG 27G2
    

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ER-rsTagRFP

    Plasmid

    #87620

    Purpose

    Endoplasmic reticulum (ER)-targeted rsTagRFP.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    ER-rsTagRFP
    

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAPV-DsRed2-FLAG-ER-5

    Plasmid

    #170622

    Purpose

    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PRLSS-SH2-KDEL

    Plasmid

    #209716

    Purpose

    Mammalian expression of SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-SH2-KDEL
    

    Tags

    PRLSS

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQIq_MRGS_HIS8_( E3_5)_FLAG

    Plasmid

    #199612

    Purpose

    Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-FLAG E3_5 (control)
    

    Tags

    8xHis and FLAG

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL50016

    Plasmid

    #50315

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
    

    Use

    Plant expression

    Mutation

    Codon-optimised for A. thaliana. BsaI/BbsI sites …

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Venus-7aa-Cb5-pEGFP-C1

    Plasmid

    #166764

    Purpose

    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5a Rat)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. mPapaya-CytERM-N-17

    Plasmid

    #56345

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    CytERM
    

    Tags

    mPapaya

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

19. mTFP1-CytERM-N-17

    Plasmid

    #55476

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    CytERM
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pUCmini-RELA-PGK-EGFP

    Plasmid

    #228051

    Purpose

    Homologous donor template for RELA knockout expressing EGFP

    Depositor

    Yohei Sato

    Article

    Sato et al Clinical Immunology Communications, Volu

    Insert

    EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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