We narrowed to 24,045 results for: She

1. pSNAPf-H2B Control Plasmid

   Plasmid

   #101124

   Purpose

   Expression of SNAP-tagged Histone H2B in mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Insert

   histone cluster 1 H2B family member j (H2BC11 Human)
   

   Use

   Tags

   SNAP-tag (SNAPf)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGEX6P1-DEST

   Plasmid

   #119749

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CBFRE-EGFP

   Plasmid

   #17705

   Depositor

   Nicholas Gaiano

   Article

   Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

   Insert

   CBFRE
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-N-HA

   Plasmid

   #119756

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS2-YFP

   Plasmid

   #27122

   Depositor

   Robert Singer

   Article

   Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

   Insert

   MS2
   

   Use

   Tags

   EYFP, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-FLAG-mCherry-C1-centrin-1

   Plasmid

   #73333

   Purpose

   Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

   Depositor

   Manuel Thery

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-Traptavidin

   Plasmid

   #24753

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 Apr 11. ():.

   Insert

   Traptavidin
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G an…

   Promoter

   Available since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

   Plasmid

   #51086

   Purpose

   Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa 1.3 kb

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET23a_mod_HuD_FL

   Plasmid

   #65751

   Purpose

   plasmid for bacterial overexpression of HuD

   Depositor

   Thomas Tuschl

   Insert

   ELAVL4 (ELAVL4 Human)
   

   Use

   Tags

   6X His and FLAG HA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAF1L

    Plasmid

    #39014

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1L (TAF1L Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73039

    Purpose

    Express Nluc, copGFP dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin + CMV enhancer and chicken bet…

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. hPAX7-3xHA-IRES-hrGFP (#400)

    Plasmid

    #78338

    Purpose

    Overexpression of human PAX7 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    PAX7 (PAX7 Human)
    

    Use

    Tags

    3x HA tag and IRES-hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pINDUCER20-hMYOD1-3xHA (#730)

    Plasmid

    #78328

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Expression

    Mutation

    Promoter

    TRE2

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAP3K15-TAP

    Plasmid

    #69728

    Purpose

    Express tandem tagged MAP3K15 in cells

    Depositor

    Daniel Liebler

    Insert

    Apoptosis signal-regulating kinase 3 (MAP3K15 Human)
    

    Use

    Tags

    HA-Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51082

    Purpose

    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB265

    Plasmid

    #69748

    Purpose

    Retroviral expression of HURP

    Depositor

    Iain Cheeseman

    Insert

    HURP (DLGAP5 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

17. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid

    #68705

    Purpose

    Francisella tularensis subsp. novicida Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-V5-SBP-C1-HshnRNPM

    Plasmid

    #64924

    Purpose

    Expresses V5- and SBP-tagged hnRNPM in mammalian cells

    Depositor

    Elisa Izaurralde

    Insert

    hnRNPM (HNRNPM Human)
    

    Use

    Tags

    V5 and SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM1579

    Plasmid

    #40240

    Depositor

    Susan Golden

    Article

    Andersson et al Methods Enzymol. 2000;305:527-42.

    Insert

    Km Resistance, neutral site II integration platform for Synechococcus
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. hMYOD1-3xHA-IRES-hrGFP (#343)

    Plasmid

    #78327

    Purpose

    Overexpression of human MYOD1 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Tags

    3x HA tag and IRES-hrGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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