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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 4341 - 4360 of 18370 results

  1. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid
    #196716
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-TagBFP-KRAB(ZIM3)
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196716-plasmid-map-sequence-id-388040

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  2. pSCM167-Flag-hSFRP1

    Plasmid
    #189538
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    Oct. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    189538-plasmid-map-sequence-id-374368

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  3. pLV-BDNF-pHluorin

    Plasmid
    #187193
    Purpose
    To express the mouse BDNF tagged with the FP pHluorin
    Depositor
    Pantelis Tsoulfas
    Insert
    BDNF (Bdnf Mouse)
    Use
    Lentiviral
    Tags
    pPHluorin
    Expression
    Mammalian
    Mutation
    The codon were optimized for better expression
    Promoter
    CMV
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    187193-plasmid-map-sequence-id-373713

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  5. p663-UBC-V5-CDK13_IDG-K

    Plasmid
    #135268
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    135268-plasmid-map-sequence-id-269140

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  6. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  9. pcDNA3.1(+)-ER-CaNARi

    Plasmid
    #64852
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNARi
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64852-plasmid-map-sequence-id-109677

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  12. mEos3.2-Farnesyl-5

    Plasmid
    #57456
    Purpose
    Localization: Membrane, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Farnesyl (HRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    encodes final 20 aa of NM_001130442.1
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57456-plasmid-map-sequence-id-393325

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  14. tdTomato-MAPTau-N-10

    Plasmid
    #58113
    Purpose
    Localization: Microtubules, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    MAPTau (MAPT Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. mCerulean-PCNA-19-SV40NLS-4

    Plasmid
    #55386
    Purpose
    Localization: Nucleus, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3xSV40 NLS and mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. mApple-MAPTau-C-10

    Plasmid
    #54924
    Purpose
    Localization: Microtubules, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    MAPTau (MAPT Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pDON-AI-Npm2(N533)

    Plasmid
    #51475
    Purpose
    Express mouse phosphorylation-mimicking Npm2 (p-Npm, N533)
    Depositor
    Shunsuke Ishii
    Insert
    Nucleoplasmin 2 (Npm2 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Eleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …
    Available Since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51475-plasmid-map-sequence-id-78547

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  19. GSK-3beta S9A (Human)/pCS2+

    Plasmid
    #16334
    Depositor
    Peter Klein
    Insert
    glycogen synthase kinase-3 beta (GSK3B Human)
    Use
    Vertebrate expression
    Tags
    Myc
    Mutation
    GSK-3 beta with serine-9 to alanine point mutation
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-CAG-GFP (AAV9-X1.1)

    Viral Prep
    #37825-AAV9-X1.1
    Purpose
    Ready-to-use AAV9-X1.1 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the AAV9-X1.1 serotype, which permits efficient transduction of brain endothelial cells. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Promoter
    CAG
    Tags
    GFP
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4341 - 4360 of 18370 results