We narrowed to 13,230 results for: CRISPR-Cas9

1. pCF712

   Plasmid

   #121663

   Purpose

   U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   Cas9(C)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. LC-E75

   Bacterial Strain

   #115925

   Purpose

   Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

3. BPK764

   Plasmid

   #65767

   Purpose

   Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-ECFP

   Plasmid

   #112220

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   ECFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK2101

   Plasmid

   #65770

   Purpose

   Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized SaCas9, and SaCas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS-3xFlag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB70

   Plasmid

   #47943

   Purpose

   Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDonor_mU6

   Plasmid

   #69350

   Purpose

   sgRNA scaffold and mouse U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   mU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mU6 promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDonor_hU6

   Plasmid

   #69312

   Purpose

   sgRNA scaffold and human U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   hU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   human U6 promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor_sU6

   Plasmid

   #69351

   Purpose

   sgRNA scaffold and synthetic U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   sU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   synthetic U6 promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6>sgRNA(F+E)

    Plasmid

    #59986

    Purpose

    Empty vector for sgRNA cloning (F+E modified backbone)

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMZ284

    Plasmid

    #52225

    Purpose

    Expression of sgRNA precursor targeted against ade6+

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAH233

    Plasmid

    #121144

    Purpose

    Expression of gRNA with BbsI placeholder for target cloning

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Insert

    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMZ285

    Plasmid

    #52226

    Purpose

    Expression of sgRNA precursor targeted against ade6M210

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ286

    Plasmid

    #52227

    Purpose

    Expression of sgRNA precursor targeted against ade6L469

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMC00023 (aka pRC0434)

    Plasmid

    #195309

    Purpose

    Lentiviral expression vector derived from LEGO-G2 with G418/Neomycin selection

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid

    #99690

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

    Plasmid

    #99694

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210749

    Purpose

    Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    CjSp D8A Cas9
    Bilbo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

    Plasmid

    #199483

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mKate2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

    Plasmid

    #199482

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mKate2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge