We narrowed to 10,183 results for: yeast

1. pMT1026

   Plasmid

   #228299

   Purpose

   Constitutive expression of PhlTA mutant with weak promoter and terminator

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   phlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   E41G

   Promoter

   KpYPT1 promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT478

   Plasmid

   #228286

   Purpose

   Constitutive mutant rPhlTA expression with strong promoter

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   rphlTA mutant
   

   Use

   Synthetic Biology

   Tags

   Three copies of VP16 (VP48)-Nuclear localization …

   Expression

   Yeast

   Mutation

   P5S, S6P, K86T, F109L, Q117R, E143K

   Promoter

   KpGAPDH promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

   Plasmid

   #225228

   Purpose

   Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

   Inserts

   Aga2
   Htt (25Q)
   

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

   Plasmid

   #225229

   Purpose

   Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

   Inserts

   Aga2
   Htt (46Q)
   

   Tags

   HA Tag and c-myc Tag

   Expression

   Yeast

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. BS-ZJLEU-PTEF1-GFP

   Plasmid

   #207018

   Purpose

   Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   GFP
   

   Expression

   Yeast

   Promoter

   pTEF1

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. PTPI1-Dsred-URA3

   Plasmid

   #207017

   Purpose

   Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   DsRed-Express 2
   

   Expression

   Yeast

   Promoter

   pTPI1

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP

   Plasmid

   #207024

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   D384A Y387A

   Promoter

   pTEF1

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP

   Plasmid

   #207023

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A I375A

   Promoter

   pTEF1

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. BS-ZJLEU-PTEF1-HFL1-Y387A-GFP

   Plasmid

   #207022

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Y387A

   Promoter

   pTEF1

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. BS-ZJLEU-PTEF1-HFL1-D384A-GFP

    Plasmid

    #207021

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    D384A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BS-ZJLEU-PTEF1-HFL1-I375A-GFP

    Plasmid

    #207020

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    I375A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

    Plasmid

    #207019

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ClhN-P1K-3VSV-Atg8-MET15

    Plasmid

    #207055

    Purpose

    Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xVSV

    Expression

    Yeast

    Promoter

    pATG8

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. P1K-3HA-Atg8∆GR-MET15

    Plasmid

    #207033

    Purpose

    Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    ∆GR

    Promoter

    pATG8

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBT9-γ1 ear

    Plasmid

    #199177

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

    Insert

    AP-1 γ1 ear domain (Ap1g1 Mouse)
    

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGAD424-Rabex-5

    Plasmid

    #197043

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    Rabex-5 (RABGEF1 Bovine)
    

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEFEX2-A2aGH

    Plasmid

    #160544

    Purpose

    pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEFEX3-A2aGH

    Plasmid

    #160545

    Purpose

    pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFEX2-A2aGH

    Plasmid

    #160547

    Purpose

    pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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