Skip to main content
Addgene
Showing: 1051 - 1100 of 1384 results

  8. pAAV-MCU-tDimer

    Plasmid
    #181865
    Purpose
    Expresses tDimer-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV and Adenoviral
    Tags
    tDimer
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181865-plasmid-map-sequence-id-355948

    Enlarge

  9. R-Q111R-N122D

    Plasmid
    #167178
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-R-Q111R-N122D
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed R at 111 to Q and changed D at 122 to N
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167178-plasmid-map-sequence-id-334483

    Enlarge

  20. PGK-HA-PGRN-LAMP1TM

    Plasmid
    #234873
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234873-plasmid-map-sequence-id-469050

    Enlarge

  24. RAT +D122H

    Plasmid
    #187426
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al bioRxiv 2021.11.29.470343
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed aspartic acid at 129 for histidine
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    187426-plasmid-map-sequence-id-374214

    Enlarge

  25. RAT +R111E

    Plasmid
    #187425
    Purpose
    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al bioRxiv 2021.11.29.470343
    Inserts
    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    Use
    Tags
    Expression
    Insect
    Mutation
    changed arginine at 118 to glutamic acid
    Promoter
    PH and p10
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    187425-plasmid-map-sequence-id-374210

    Enlarge

  26. S+Q111R+N122D

    Plasmid
    #167175
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+Q111R+N122D
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R and changed N at 122 to D
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167175-plasmid-map-sequence-id-334477

    Enlarge

  27. S+10subs

    Plasmid
    #167176
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+10R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed A at 112 to T; E at 116 to D; I at 135 to…
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167176-plasmid-map-sequence-id-345188

    Enlarge

  28. S+12subs

    Plasmid
    #167177
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-S+12R
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed Q at 111 to R; A at 112 to T; E at 116to …
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167177-plasmid-map-sequence-id-334485

    Enlarge

  29. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

    Enlarge

  30. pLentiCMV_HyPer7RgDAAO

    Plasmid
    #217653
    Purpose
    Expresses the fusion of HyPer7,a H2O2 sensor, and Rhodotorula gracilis D amino acid oxidase (DAAO) to measure transport of D amino acids across the plasma membrane
    Depositor
    Seth Parker
    Article
    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi
    Insert
    HyPer7 D amino acid oxidase
    Use
    Lentiviral
    Tags
    Nuclear export signal
    Expression
    Mammalian
    Mutation
    Fused DAAO to the C-terminus of HyPer7 using a Gl…
    Promoter
    CMV
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217653-plasmid-map-sequence-id-441459

    Enlarge

  46. pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID

    Plasmid
    #122981
    Purpose
    AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    Syph-GFP-myc-BoNT/B(1-146)-iLID (Syp Rat, Synthetic)
    Use
    AAV
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122981-plasmid-map-sequence-id-238426

    Enlarge

  47. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

    Enlarge

  48. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

    Plasmid
    #111396
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    DTR-EGFP
    Use
    AAV
    Tags
    EGFP and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111396-plasmid-map-sequence-id-212262

    Enlarge

  50. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

    Plasmid
    #122982
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-BoNT/B(1-146)-iLID
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    iLID contains long lived V416I mutation
    Promoter
    human synapsin
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122982-plasmid-map-sequence-id-238428

    Enlarge

Showing: 1051 - 1100 of 1384 results