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Showing: 421 - 440 of 1425 results

  5. pCAGGS-SMS1-TEV-Twin-Strep

    Plasmid
    #202528
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202528-plasmid-map-sequence-id-406371

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  6. pMX-CAG-CD3-P2A-CD90.2

    Plasmid
    #163336
    Purpose
    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the CAG promoter
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    CD3
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163336-plasmid-map-sequence-id-322032

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  14. pCAGGS-SMS2-TEV-Twin-Strep

    Plasmid
    #202529
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS2 (SGMS2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TGA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202529-plasmid-map-sequence-id-399646

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  15. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102885-plasmid-map-sequence-id-259794

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  18. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid
    #234724
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.
    Depositor
    Kirill Martemyanov
    Article
    Zhao et al Nature Communications 2025
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    T2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG promoter
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234724-plasmid-map-sequence-id-466565

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Showing: 421 - 440 of 1425 results