Addgene

We narrowed to 6,715 results for: SIM;

Showing: 421 - 440 of 6715 results

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pCMVmyc-ORF73SOCS

Plasmid

#21546

Depositor

J Simas

Article

Rodrigues et al EMBO J. 2009 May 6. 28(9):1283-95.

Insert

ORF73 (ORF73 MuHV-4)

Use

Tags

Myc

Expression

Mammalian

Mutation

amino acids at positions 199, 202, 203 and 206 we…

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pMA4008

Plasmid

#70109

Purpose

mtDNA destruction/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
Herpes simplex virus UL12.5M185

Use

Tags

none

Expression

Mammalian

Mutation

N-terminal deletion of UL12.5

Promoter

CMV and RSV

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A2_F

Plasmid

#237351

Purpose

Expression plasmid for RSV A2 F

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV A2 F human codon optimized from Genbank Accesion QGW56794.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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PfICH pET15b

Plasmid

#60684

Purpose

Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

Depositor

Mark Wilson

Article

Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

Insert

inhA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
HDM_RSV_A_1982_F

Plasmid

#237355

Purpose

Expression plasmid for RSV A F from 1982

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV A F from 1982 human codon optimized from Genbank Accesion KJ723472

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A_2020_F

Plasmid

#237356

Purpose

Expression plasmid for RSV A F from 2020

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV A F from 2020 human codon optimized from Genbank Accesion PP495954

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-EB1

Plasmid

#17234

Depositor

Lynne Cassimeris

Article

Piehl et al Mol Biol Cell. 2003 Mar . 14(3):916-25.

Insert

EB1 (MAPRE1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2008

Availability

Academic Institutions and Nonprofits only

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stathmin-GFP

Plasmid

#86782

Purpose

Mammalian expression of stathmin-GFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD011

Plasmid

#116852

Purpose

Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
mScarletI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDAS12069_U6-pegRNA-mCherry

Plasmid

#177180

Purpose

pegRNA cloning plasmid with mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1938

Plasmid

#82563

Purpose

High copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

Ple261

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pDAS12222_U6-pegRNA-BFP

Plasmid

#177181

Purpose

pegRNA cloning plasmid with BFP marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1982

Plasmid

#49113

Purpose

AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

ssAAV-Ple261-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CLDN5

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Phi3-iRFP

Plasmid

#106296

Purpose

CMV driven iRFP with n terminal membrane binding domain of GAP43

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

iRFPmem

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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psAAVS1-T2

Plasmid

#60543

Purpose

GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

Depositor

William Simonds

Article

Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

Insert

ProtoSpacer AAVS1-T2 (AAVS1 Human)

Use

CRISPR; Kan

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 pET15b

Plasmid

#60677

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
stathmin-CFP

Plasmid

#86783

Purpose

Mammalian expression of stathmin-CFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p15aC-1D-5

Plasmid

#107865

Purpose

Bacterial expression of PIS and PI4P5K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

proD and proD (in operon)

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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Gag-delta-p6-mEGFP

Plasmid

#116890

Purpose

expresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

synthetic Gag missing the p6 domain

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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