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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,310 results for: pho;

Showing: 421 - 440 of 9310 results

  1. pEHIShqprt

    Plasmid
    #92166
    Purpose
    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector
    Depositor
    Huanting Liu, James Naismith
    Article
    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E
    Insert
    Quinolinate Phosphoribosyltransferase (QPRT Human)
    Use
    Tags
    TEV protease -cleavable N-terminal 6His
    Expression
    Bacterial
    Mutation
    changed Asp 2 to Asn to create BspHI site
    Promoter
    T7
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    92166-plasmid-map-sequence-id-190226

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  8. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  14. pPB.DEST-Illusia(YYEE)

    Plasmid
    #222594
    Purpose
    Piggybac expression vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(YYEE)
    Use
    Transposon-based stable expression
    Tags
    Expression
    Mammalian
    Mutation
    Mutations in the NPxY sites equivolent to Y783E, …
    Promoter
    CAG
    Available since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222594-plasmid-map-sequence-id-450070

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  16. pPB.DEST-Illusia(Y783F)

    Plasmid
    #222596
    Purpose
    Piggybac expression vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(Y783F)
    Use
    Transposon-based stable expression
    Tags
    Expression
    Mammalian
    Mutation
    Mutation in the NPxY(783) site equivolent to Y783…
    Promoter
    CAG
    Available since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222596-plasmid-map-sequence-id-450084

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  18. pPB.DEST-Illusia(Y795F)

    Plasmid
    #222598
    Purpose
    Piggybac expression vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(Y795F)
    Use
    Transposon-based stable expression
    Tags
    Expression
    Mammalian
    Mutation
    Mutation in the NPxY(795) site equivolent to Y795…
    Promoter
    CAG
    Available since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222598-plasmid-map-sequence-id-450087

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Showing: 421 - 440 of 9310 results