We narrowed to 1,712 results for: plasmid for e coli

1. pJL017

   Plasmid

   #53532

   Purpose

   This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

   Depositor

   James Fleckenstein

   Article

   Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

   Insert

   etpBA
   

   Use

   Tags

   6His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDL14

   Plasmid

   #106901

   Purpose

   pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   mutS
   lacI
   rhaR
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lysine 622 was replaced by Alanine

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4092694

   Plasmid

   #153907

   Purpose

   Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | t7 consensus from NEB, G is +1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDOC-K-glmS-lacIZ

   Plasmid

   #158061

   Purpose

   Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   lacIZ operon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRRNAcos

   Plasmid

   #78492

   Purpose

   Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

   Depositor

   David Bikard

   Article

   Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

   Insert

   tracrRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRAD1

   Plasmid

   #47697

   Purpose

   General cloning vector for use in E. coli or D. radiodurans

   Depositor

   Mary Lidstrom

   Article

   Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   repU

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRADZ1

   Plasmid

   #47698

   Purpose

   General cloning vector for use in E. coli or D. radiodurans with lacZ

   Depositor

   Mary Lidstrom

   Article

   Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   repU

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL_dstβL

   Plasmid

   #73805

   Purpose

   Plasmid for selection for insertion and homodimerization

   Depositor

   Sarel Fleishman

   Article

   Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

   Inserts

   CAT Marker
   ToxR
   beta lactamase
   spectomycin resistance
   CLS TM domain
   

   Use

   Tags

   Part Of the dstbl chiemra, Part of the dsTbL chim…

   Expression

   Bacterial

   Mutation

   Promoter

   NaN and ToxR promotor

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pDEINO3

   Plasmid

   #179487

   Purpose

   Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

   Depositor

   Bogumil Karas

   Article

   Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

   Insert

   TetR/A
   

   Use

   Synthetic Biology; Bacterial and yeast cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXD70LacZ3-Pcsd-LacZ

    Plasmid

    #191619

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoter

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD70LacZ4-PdegV-LacZ

    Plasmid

    #191620

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoter

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTE1069

    Plasmid

    #186629

    Purpose

    SCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.

    Depositor

    Eriko Takano

    Article

    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

    Inserts

    scbR
    scbAp promoter
    

    Use

    Synthetic Biology

    Tags

    6xArg

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23100 and scbAp

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEVA3b61

    Plasmid

    #58310

    Purpose

    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p007ampGFP

    Plasmid

    #58535

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5 between two lac operators

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5 between two lac operators

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE30del

    Plasmid

    #106902

    Purpose

    pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC-GFP-AT

    Plasmid

    #133306

    Purpose

    "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    axe/txe toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC-GFP-HS

    Plasmid

    #133305

    Purpose

    "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    hok/sok toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNO4

    Plasmid

    #101066

    Purpose

    BphP1-PpsR2 reporter plasmid; PBr_crtE-sfGFP

    Depositor

    Jeffrey Tabor

    Article

    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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