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pKH032

Plasmid

#224548

Purpose

2A-3xNLS-2xClover mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

2xClover

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKH040

Plasmid

#224549

Purpose

2A-3xNLS-tdTomato mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

tdTomato

Use

CRISPR and Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pYO286

Plasmid

#235730

Purpose

Protein expression of yeast VPS15 (1-981)-ZZ in yeast

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

aa 1-981

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRD4

Plasmid

#183331

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRD4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRD4

Use

Lentiviral

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG013

Plasmid

#231334

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid with FRT site. L1-p15A-Gm-FRT-ELT4; GG assembly from pNDGG021, pOGG009, pNDGG006, pNDGG009, pOGG014. NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG001

Plasmid

#231313

Purpose

Vector part for BEVA; BEVA2.0 Position 4 I-SceI recognition/cut site, SpR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 4 I-SceI recognition/cut site, SpR.

Expression

Bacterial

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.SynFLEX.(cyto).cpSFGFP.mIRFP670nano3

Plasmid

#244918

Purpose

cpSFGFP control with non-responsive mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.mIRFP670nano3

Use

AAV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

Plasmid

#244836

Purpose

Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS

Plasmid

#244839

Purpose

Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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GST-Thrombin-Munc13-1 MUN domain (aa859-1516 delta1408-1452 EF)

Plasmid

#243818

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

GST

Expression

Bacterial

Mutation

1408-1452 --> EF

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

Plasmid

#244843

Purpose

Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Lbm6-Cas12a

Use

CRISPR

Tags

10xHis-MBP and 4xSV40 NLS

Expression

Bacterial

Mutation

D535G/S551F/D665N

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-empty

Plasmid

#239099

Purpose

pGL3 luciferase vector with no promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α

Plasmid

#239097

Purpose

Empty pcDNA4-C backbone with EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Type

Empty backbone

Expression

Mammalian

Promoter

EF1α

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mem-mScarlet-ORF-3

Plasmid

#241185

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mem-mScarlet-ORF-2

Plasmid

#241184

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mem-mScarlet-ORF-1

Plasmid

#241183

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mNeongreen-ORF1

Plasmid

#241208

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-lifeact-mNeongreen-ORF-1

Plasmid

#241204

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mNeongreen-ORF2

Plasmid

#241209

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mNeongreen-ORF3

Plasmid

#241210

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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