We narrowed to 13,280 results for: cDNA

1. pcDNA3.1-SARS-CoV-2-S (D614G)

   Plasmid

   #215838

   Purpose

   Expresses SARS-CoV-2 Spike protein in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 Spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-FLAG-HA-CAD S1859A

   Plasmid

   #46239

   Purpose

   FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1859 changed to Alanine (S1859A)

   Promoter

   cmv

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3.1 (-) + codon optimized Brn3a

   Plasmid

   #86829

   Purpose

   mammalian expression of the transcription factor Brn3a

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   Brn3a (POU4F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-mCherry-Rheb(C181S)

   Plasmid

   #192437

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-Rheb(C181S) (RHEB Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO HIS DNAJA1

   Plasmid

   #19545

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1/V5-His-TOPO-mir17-92

   Plasmid

   #21109

   Depositor

   Joshua Mendell

   Article

   O'Donnell et al Nature. 2005 Jun 9. 435(7043):839-43.

   Insert

   miR-17-92 cluster (MIR17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA4-TO-Hygromycin-mVenus-MAP

   Plasmid

   #44099

   Depositor

   Dannel McCollum

   Article

   Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

   Type

   Empty backbone

   Use

   Tags

   mVenus-MAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5/FRT/TO GFP DNAJA1

   Plasmid

   #19491

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJA1 (DNAJA1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-FRT/TO-mNeonGreen-BRD4

   Plasmid

   #204692

   Purpose

   This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid

    #89637

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO-GAlpha11-RLuc8

    Plasmid

    #140983

    Purpose

    Encodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha11-RLuc8 (GNA11 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AIRAP 9: PSMD2.GST.V2.pcDNA3

    Plasmid

    #21799

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD2 (PSMD2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3 Flag TSC2 (T1462A)

    Plasmid

    #14130

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 T1462A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T1462A

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

    Plasmid

    #66789

    Purpose

    Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

    Depositor

    Lotte Pedersen

    Article

    Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

    Insert

    PDGFRalpha (Pdgfra Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D842V (constitutively active)

    Promoter

    EF1a

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-ELMOD2-myc-His6

    Plasmid

    #201901

    Purpose

    Used for expression of human protein ELMOD2 in mammalian cells

    Depositor

    Richard Kahn

    Insert

    ELMOD2 (ELMOD2 Human)
    

    Use

    Tags

    myc, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

    Plasmid

    #159455

    Purpose

    Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

    Depositor

    Oliver Griesbeck

    Article

    Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

    Insert

    mCRISPRed
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1/Myc-His(-)-MSulf-1

    Plasmid

    #13007

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    mouse sulfatase 1 (Sulf1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. GeNL(Ca2+)_520/pcDNA3

    Plasmid

    #85204

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_520
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Human NCKX3-FlagTag (pcDNA3.1-)

    Plasmid

    #75206

    Purpose

    Mammalian expression of SLC24A3

    Depositor

    Jonathan Lytton

    Article

    Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

    Insert

    SLC24A3 (SLC24A3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge