We narrowed to 5,618 results for: cre encoding plasmid

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pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

Plasmid

#105035

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

Plasmid

#105017

Purpose

Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Kcmf1 (Kcmf1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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564aa ELL2 bluescript

Plasmid

#127267

Purpose

For in vitro translation of human ELL2 lacking COOH region

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

Addgene Plasmid 127256--SphI site filled in, then…

Promoter

T7

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

Plasmid

#121804

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

Plasmid

#121808

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-Notch1 3'UTR-mir200c-mut

Plasmid

#84596

Purpose

Renilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

NOTCH1 3'UTR (NOTCH1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on NOTCH1 3…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCIneoRL-LFNG 3'UTR-mir200c-mut

Plasmid

#84594

Purpose

Renilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding site

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

LFNG 3'UTR (LFNG Human)

Use

Luciferase

Tags

Renilla Luciferase

Mutation

Mutation on miR-200c binding site on LFNG 3'…

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

Plasmid

#64216

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBZ-mRuby2

Plasmid

#110650

Purpose

expresses a PAR-binding (PBZ) domain fused to mRuby2

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

mRuby2

Expression

Mammalian

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAcBac3-EcLeu TAG-EGFP**

Plasmid

#228785

Purpose

EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcLeuRS
EcLtR
EGFP**

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBiFC-PBZ-VN

Plasmid

#110646

Purpose

expresses a PAR-binding (PBZ) domain fused to venus N-terminus

Depositor

Chris Lord

Article

Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

Insert

PBZ domain from APLF, amino acids 371-451 (APLF Human)

Tags

DDK and VN

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF4_P2A_Hygro_Barcode

Plasmid

#120455

Purpose

Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltaMBII_P2A_Hygro_Barcode

Plasmid

#120505

Purpose

Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

c-MYC deltaMBII (MYC Human)

Use

Lentiviral

Mutation

Deletion of MYC Box II domain

Promoter

EF1a

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

Plasmid

#105040

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

Plasmid

#105027

Purpose

Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stat3 (Stat3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

Plasmid

#105022

Purpose

Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Apc (Apc Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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