We narrowed to 6,429 results for: rel

1. hTln1-R12DD-SCLS

   Plasmid

   #191443

   Purpose

   expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

   Depositor

   Ben Goult

   Article

   Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

   Insert

   Human Talin1 R12DD (TLN1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   deletion between residues K2375 and A2397

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-FLAG3-drN-hsAgo2

   Plasmid

   #221724

   Purpose

   Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drN-hsAgo2 (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Swap of N-domain for zebrafish Ago2 N-domain

   Promoter

   simian CMV IE94

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2+-FLAG3-hsN-drAgo2

   Plasmid

   #221717

   Purpose

   Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsN-drAgo2 (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Swap of N-domain for human Ago2 N-domain

   Promoter

   simian CMV IE94

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNK7186

   Plasmid

   #219758

   Purpose

   Vector for expression PpASCL in mammalian cells (HEK293NT)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PpASCL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2+-FLAG3-hsAgo2EF-DY

   Plasmid

   #221723

   Purpose

   Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2EF-DY (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   E637D and F666Y in active site

   Promoter

   simian CMV IE94

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG_SRGAP2A_SH3(W765A)_GFP

   Plasmid

   #222616

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG_SRGAP2A_SH3(W765A)_HA

   Plasmid

   #222614

   Purpose

   Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Point mutation W765A (SH3 domain)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG SRGAP2A_EVH1(P339L F342C)

   Plasmid

   #222613

   Purpose

   Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations P339L and F342C

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB-EF1A-Puro-mCherry-RAB7-S72A

   Plasmid

   #221556

   Purpose

   Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Change serine 72 to alanine

   Promoter

   EF1a

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNK7191

    Plasmid

    #219764

    Purpose

    MoClo-compatible Level M for expression PpASCL in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNK7182

    Plasmid

    #219767

    Purpose

    Vector for expression PpASCL in yeast (P.pastoris)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmcherry-mTet1s pc3905

    Plasmid

    #201703

    Purpose

    Expresses of mcherry-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3-TEX264-SIM1_mut-cFlag

    Plasmid

    #220205

    Purpose

    Mammalian expression of TEX264 with SIM1 motif mutated, C-terminal Flag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

    Promoter

    CMV

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA3-TEX264-SIM2_mut-cFlag

    Plasmid

    #220206

    Purpose

    Mammalian expression of TEX264 with SIM2 motif mutated, C-terminal Flag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

    Promoter

    CMV

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3-TEX264-SHP1mut-cFlag

    Plasmid

    #220208

    Purpose

    Mammalian expression of TEX264 with SHP domain mutated, C-terminal Flag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    p.280GEGPL>RERPA; c.838G>C,844G>A,850T&g…

    Promoter

    CMV

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA5-TEX264-SIM2_mut-cGFP

    Plasmid

    #220213

    Purpose

    Mammalian expression of TEX264 with SIM2 motif mutated, fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINOH11-A10

    Plasmid

    #184278

    Purpose

    Expresses nanobody H11-A10 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-A10
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOPINO_H11-B5

    Plasmid

    #184279

    Purpose

    Expresses nanobody H11-B5 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-B5
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPINO_11-H6

    Plasmid

    #184280

    Purpose

    Expresses nanobody H11-H6 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-H6
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge