We narrowed to 15,989 results for: LEA

1. pcDNA3-PM-deltaCKAR

   Plasmid

   #222429

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Plasma membrane delta-selective C kinase activity reporter
   

   Tags

   CFP and YFP

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. esgRNA_SlMIR164b

   Plasmid

   #231153

   Purpose

   T-DNA encoding TRV2 with mobile gRNA targeting SlMIR164b

   Depositor

   Daniel Voytas

   Article

   Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

   Insert

   mobile gRNA targeting SlMIR164b
   

   Expression

   Plant

   Promoter

   PEBV sub-genomic

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-PHOSPHO2-TEV-Twin-Strep

   Plasmid

   #222016

   Purpose

   Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO2 (PHOSPHO2 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-PHOSPHO2-TEV-Twin-Strep

   Plasmid

   #222017

   Purpose

   Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO2 (PHOSPHO2 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Bacterial

   Mutation

   deleted stop codon (TAA)

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac SOX R139A

   Plasmid

   #192058

   Purpose

   Expression vector for SOX (ORF37) R139A

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37)
   

   Expression

   Insect

   Mutation

   R139A

   Promoter

   Polyhedrin

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACE WT SOX pACEmam1

   Plasmid

   #192049

   Purpose

   Expresses WT SOX (pACEmam1)

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   WT SOX
   

   Use

   Unspecified

   Promoter

   CMV

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac SOX Q129H

   Plasmid

   #192057

   Purpose

   Expression vector for SOX (ORF37) Q129H

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37) Q129H
   

   Expression

   Insect

   Mutation

   Q129H

   Promoter

   Polyhedrin

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Nuc-deltaCKAR

   Plasmid

   #222430

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Nucleus delta-selective C kinase activity reporter
   

   Tags

   CFP and YFP

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. T2A GFP H2B

   Plasmid

   #227722

   Purpose

   Plasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Oliver Hobert, Haosheng Sun

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   (nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   T2A 3xNLS GFP

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sc++

    Plasmid

    #198545

    Purpose

    Expresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    Sc++
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpG

    Plasmid

    #198543

    Purpose

    Expresses human codon-optimized SpG and blasticidin resistance: EFS promoter-SpG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Golgi-deltaCKAR

    Plasmid

    #222428

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Golgi delta-selective C kinase activity reporter
    

    Tags

    CFP and YFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbA5c-cdl

    Plasmid

    #207649

    Purpose

    Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    cdl
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-cdl

    Plasmid

    #207652

    Purpose

    Overexpression of heterologous L-cysteine desulfidase in E. coli MG1655

    Depositor

    Benjamin Woolston

    Article

    Hayes et al Cell Rep. 2023 Dec 26;42(12):113481. doi

    Insert

    cdl
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDLterm

    Plasmid

    #211903

    Purpose

    Base plasmid for dual-luciferase constructs to measure terminator strength

    Depositor

    Stan Fields

    Article

    Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Plant

    Promoter

    35S enhancer + 35S minimal promoter

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-HGM (optimized)

    Plasmid

    #220988

    Purpose

    Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

    Depositor

    Joshua Kritzer

    Article

    Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

    Insert

    HaloTag-GFP-mito
    

    Use

    AAV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid

    #221844

    Purpose

    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

    Depositor

    Stephen Terry

    Article

    O’Sullivan et al bioRxiv 2024.03.04.583298

    Inserts

    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    

    Use

    CRISPR; Tol2 transposon optimised for chick expre…

    Expression

    Mammalian

    Promoter

    GACG and U6.3 chick

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGK43

    Plasmid

    #222223

    Purpose

    Edits NPAS2 Gene.

    Depositor

    Seth Shipman

    Article

    Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

    Insert

    Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTP489

    Plasmid

    #216366

    Purpose

    xNup155-Nb1t (+3Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xNup155-Nb1t (+3Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSC263

    Plasmid

    #216367

    Purpose

    xhNup155-Nb2i Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup155-Nb2i
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-Avi-SUMOEu1

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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