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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,189 results for: LEA

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Showing: 4401 - 4420 of 12189 results

  3. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Publication
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  4. SpCas9-NRCH-YE1-BE4max

    Plasmid
    #198549
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198549-plasmid-map-sequence-id-388903

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  12. pGBW-m4133850

    Plasmid
    #152865
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152865-plasmid-map-sequence-id-320764

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  13. pGBW-m4134285

    Plasmid
    #152413
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152413-plasmid-map-sequence-id-324513

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  14. XClone-Cas9-Cronus-T2A-BFP

    Plasmid
    #141209
    Purpose
    Tunable and temporal expression control of Cas9
    Depositor
    Rory Johnson
    Insert
    Cas9
    Expression
    Mammalian
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141209-plasmid-map-sequence-id-299562

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  15. pGBW-m4133424

    Plasmid
    #152851
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152851-plasmid-map-sequence-id-300272

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  19. UBC64

    Plasmid
    #25143
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc64.248.414 (UBE2Q1 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains UBC domain; residues 248-414
    Available since
    Oct. 31, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4401 - 4420 of 12189 results