We narrowed to 10,362 results for: Coli
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Plasmid#170024PurposeEncodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL2082
Plasmid#155162PurposeExpresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)DepositorInsert(MlaFΔ[248-269])-(6xHis-TEV-MlaB)
ExpressionBacterialMutationdeleted amino acids 248-269 in MlaFAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1514
Plasmid#155147PurposeExpresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaDDepositorInsertmlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1965
Plasmid#155152PurposeExpresses MlaF(1-246)-(6 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1966
Plasmid#155153PurposeExpresses MlaF(1-246)-(10 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1967
Plasmid#155154PurposeExpresses MlaF(1-246)-(14 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
Mutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-2
Plasmid#128651PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-BufAI-1
Plasmid#128650PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pNeo
Plasmid#141058PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
ExpressionMammalianAvailable SinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-cimA3.7
Plasmid#134595PurposeLow Phosphate inducible mutant citramalate synthaseDepositorInsertcimA3.7
ExpressionBacterialMutationI47V, E114V, H126Q, T204A, L238SPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-β20cp6
Plasmid#127060PurposeLow phosphate inducible circular permutation of GFP, which is a substrate of the lon proteaseDepositorInsertGFPcp6-beta20
TagsHis tagExpressionBacterialPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM108
Plasmid#134160PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationKMLL, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM107
Plasmid#134159PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…Available SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY151
Plasmid#130947PurposepspFΔHTH::λN22plus gene controlled by promoter PrhaBDepositorInsertsrhaS
pspFΔHTH::λN22plus
UseSynthetic BiologyTagspspFΔHTH::λN22plusExpressionBacterialMutationThe HTH domain of the pspF gene was deleted (297-…PromoterPcon and PrhaBAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-S15
Plasmid#127821PurposeProduction of recombinant human ribosomal protein S15DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
TagssfGFPExpressionBacterialPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL829
Plasmid#119697Purposeprecursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347CDepositorInsertslow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
TagsnoneExpressionBacterialMutationmutations Y283D, G289C and V347CPromoterT5Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
TagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
Tags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only