We narrowed to 48,883 results for: des.1

1. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

   Plasmid

   #188976

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   g2 guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6P-1 Monosiga brevicollis CBS-pair domain

   Plasmid

   #166435

   Purpose

   Express pGEX-6P-1 Monosiga brevicollis CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Monosiga brevicollis CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-6P-1 Naegleria fowleri CBS-pair domain

   Plasmid

   #166437

   Purpose

   Express pGEX-6P-1 Naegleria fowleri CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Naegleria fowleri CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6P-1 Toxoplasma gondii CBS-pair domain

   Plasmid

   #166438

   Purpose

   Express pGEX-6P-1 Toxoplasma gondii CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Toxoplasma gondii CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn

   Plasmid

   #159503

   Purpose

   Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

   Depositor

   Julius Lucks

   Article

   Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

   Insert

   Termination Module:Linear-3_PolyU(1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-27

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

   Plasmid

   #21795

   Depositor

   David Ron

   Article

   Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

   Insert

   AIRAP (Zfand2a Mouse)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   amino acids 1-50

   Promoter

   Available since

   Sept. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF myc Gal cycT2b (1-420) (P#1720)

   Plasmid

   #14602

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

   Insert

   cycT2 (CCNT2 Human)
   

   Use

   Tags

   Gal and myc

   Expression

   Mammalian

   Mutation

   amino acids 1-420 of cyclinT2

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

   Plasmid

   #179002

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

   Plasmid

   #62955

   Purpose

   Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

    Plasmid

    #62908

    Purpose

    Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-dVP16AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167942

    Purpose

    Inducible NKX2-1 overexpression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    TTF1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)

    Plasmid

    #221997

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1330I, A1341V, N1342S, E1370K, T1380I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

    Plasmid

    #62914

    Purpose

    Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-p65AD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

    Plasmid

    #62897

    Purpose

    Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62903

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70

    Plasmid

    #62945

    Purpose

    Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-QF2-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGST-TEV-yVms1(1-417,delta(38-69))

    Plasmid

    #128739

    Purpose

    Bacterial overexpression and crystallization

    Depositor

    Chris Hill

    Article

    Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

    Insert

    Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 1-417 with deletion of 38-69

    Promoter

    T7

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLpC-Cat-L-IFP-F[1]+Plum (mammalian)

    Plasmid

    #52905

    Purpose

    Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Cat-L-IFP-F[1]+Plum
    

    Use

    Retroviral

    Tags

    L-IFP-F[1]

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)

    Plasmid

    #52907

    Purpose

    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[1]+Plum
    

    Use

    Retroviral

    Tags

    L-IFP-F[1]

    Expression

    Mammalian

    Mutation

    Promoter

    Unknown

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only