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We narrowed to 35,837 results for: CaS

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Showing: 4461 - 4480 of 35837 results

  6. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

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  8. loxP-T2A-Myr-Casp8-ERT2-rox

    Plasmid
    #182362
    Purpose
    Shuttle vector for T2A-Myr-Casp8-ERT2
    Depositor
    Mario Capecchi
    Insert
    loxP-T2A-Myr-Casp8-ERT2-rox
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Expression
    Mammalian
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182362-plasmid-map-sequence-id-358219

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  9. loxP-T2A-Myr-DeadCasp8-ERT2-rox

    Plasmid
    #182361
    Purpose
    Shuttle vector for T2A-Myr-DeadCasp8-ERT2
    Depositor
    Mario Capecchi
    Insert
    loxP-T2A-Myr-DeadCasp8-ERT2-rox
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Expression
    Mammalian
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182361-plasmid-map-sequence-id-358216

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  11. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

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  12. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169743
    Purpose
    CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    mScarlet-I
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169743-plasmid-map-sequence-id-334355

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  14. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

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  20. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-9

    Plasmid
    #129061
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA9 (mouseTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129061-plasmid-map-sequence-id-256271

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Showing: 4461 - 4480 of 35837 results