We narrowed to 4,733 results for: GCA

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pk335-CARGO-6mer-26kb-USF

Plasmid

#227467

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 26kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-11kb-USF

Plasmid

#227473

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 11kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-7.1kb-USF

Plasmid

#227474

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 7.1kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-3.3kb-USP

Plasmid

#227450

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 3.3kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-2.1kb-USP

Plasmid

#227451

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 2.1kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-33kb-USF

Plasmid

#227465

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 33kb Upstream Fgf5

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-26kb-USP

Plasmid

#227444

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 26kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-6mer-11kb-USP

Plasmid

#227447

Purpose

6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 11kb Upstream Prdm8

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5h

Plasmid

#160295

Purpose

Yeast CRISPR plasmid targeting the hphMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5n

Plasmid

#160296

Purpose

Yeast CRISPR plasmid targeting the natMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCY5k

Plasmid

#160294

Purpose

Yeast CRISPR plasmid targeting the kanMX cassette

Depositor

Amir Aharoni

Article

Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

Inserts

sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9

Use

CRISPR

Tags

8xHis-tag and SV40 NLS

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#1-puro

Plasmid

#231984

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#2-puro

Plasmid

#231983

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-TMEM173-ts1

Plasmid

#174283

Purpose

TMEM173 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

TMEM173 shRNA (STING1 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_003.gEZH2

Plasmid

#220318

Purpose

Expresses guide RNA for CRISPR/Cas9 knockout of EZH2 for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 gRNA (EZH2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(FOXH1_5-2)-PGKpuroBFP-W

Plasmid

#211962

Purpose

Express gRNA against FOXH1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting FOXH1 (FOXH1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ARID1A(44))-PGKpuro2ABFP-W

Plasmid

#200468

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44) (ARID1A Human)

Use

Lentiviral

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K

Plasmid

#187274

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Mutation

Anillin A740D, E758K

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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