We narrowed to 16,622 results for: puromycin

1. CCR5_gRNA_pX459

   Plasmid

   #192268

   Purpose

   gRNA expression for CRISPR Genome Editing of CCR5

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   CCR5 guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE puro human IRS-1

   Plasmid

   #11025

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJC5-LAMP1-RFP-2xFlag

   Plasmid

   #102931

   Purpose

   Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   mRFP-2xFlag

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LPC-flag-REST-WT

   Plasmid

   #41903

   Depositor

   Stephen Elledge

   Article

   Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

   Insert

   RE1-silencing transcription factor (REST Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSD080A

   Plasmid

   #218138

   Purpose

   Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD40-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK1221

   Plasmid

   #84220

   Purpose

   Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry

   Depositor

   Martin Kampmann, Jonathan Weissman

   Article

   Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. DDX5 WT V5

   Plasmid

   #199576

   Purpose

   Expresses WT DDX5 cloned into pLenti puro

   Depositor

   Michael Kastan

   Article

   McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

   Insert

   DDX5 (DDX5 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

   Plasmid

   #100157

   Purpose

   Expresses MUS81

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   MUS81 (MUS81 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MelanoTag-PURO (pLJC5-GPR143-mScarlet-3xHA)

   Plasmid

   #160369

   Purpose

   Stable expression of GPR143-mScarlet-3xHA fusion protein for the isolation of melanosomes.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   GPR143 (GPR143 Human)
   

   Use

   Lentiviral

   Tags

   mScarlet-3xHA

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. EcSTH

    Plasmid

    #211923

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMma PylT_FLAG-Mma PylRS AF

    Plasmid

    #140023

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y271A, Y349F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGreenPuro-shRNA-Stx3S-C4

    Plasmid

    #99742

    Purpose

    3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

    Insert

    shRNA against Stx3S
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-SFFV-NFE2L1-Puro

    Plasmid

    #181917

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L1 (NFE2L1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV/TO eGFP Puro (w159-1)

    Plasmid

    #17481

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-RT001-LVB

    Plasmid

    #163381

    Purpose

    Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEEGFRtm-BFP (LVB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ609-dCas9-KR-puro

    Plasmid

    #158478

    Purpose

    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

    Depositor

    Brendan Price

    Article

    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

    Insert

    dCas9-KR
    

    Use

    CRISPR

    Tags

    KillerRed

    Expression

    Mammalian

    Mutation

    Cas9m4 (D10A+D839A+H840A+N863A)

    Promoter

    CMV

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC118-mCtnnb1-FLIP-FlpE-Puro

    Plasmid

    #84541

    Purpose

    CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mouse beta-catenin FLIP-FlpE - Puro targeting vector
    

    Use

    Cre/Lox and Mouse Targeting

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only